检验医学与临床2025，Vol.22Issue(8)：1014-1018,1023,6.DOI:10.3969/j.issn.1672-9455.2025.08.002
肠道菌群丰度及血清sFas、8-OHdG对重度阿尔茨海默病的预测价值
Predictive value of intestinal flora abundance and serum sFas,8-OHdG in severe Alzheimer's disease
摘要
Abstract
Objective To investigate the predictive value of intestinal flora abundance and serum soluble apoptosis related factors(sFas)and 8-hydroxydeoxyguanosine(8-OHdG)in severe Alzheimer's disease(AD).Methods A total of 102 AD patients admitted to Hebei Yanda Hospital from May 2022 to April 2024 were selected as AD group,and 114 healthy volunteers who underwent physical examination in Hebei Yanda Hospital during the same period were selected as control group.According to the Clinical Dementia Rating Scale(CDR)score,they were divided into mild to moderate group(CDR score was 1-2 points)and severe group(CDR score was 3 points).High throughput sequencing was used to detect the abundance of intestinal flora in AD patients,and serum sFas,8-OHdG,interleukin(IL)-10 and IL-1β levels were detected by enzyme-linked immunosorbent assay.Pearson correlation was used to analyze the correlation between serum sFas,8-OHdG levels and intestinal flora abundance in AD patients.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of serum sFas and 8-OHdG in severe AD.Results Serum sFas and 8-OHdG levels in AD group were higher than those in control group,and the differences were statistically sig-nificant(P<0.05).There were 75 patients in the mild to moderate group and 27 patients in the severe group.The Simple Intelligent Mental Transformation Quantitative Change Scale score of the severe group was lower than that of the mild to moderate group,with statistical significance(P<0.05).Serum sFas,8-OHdG and IL-1β levels in the severe group were higher than those in the mild to moderate group,and serum IL-10 level in the severe group was lower than that in the mild to moderate group,with statistical significance(P<0.05).The abundance of Escherichia coli and Staphylococcus bacteria in the severe group was higher than that in the mild to moderate group,while the abundance of Bifidobacterium and Lactobacillus bacteria was lower than that in the mild to moderate group,with statistical significance(P<0.05).Pearson correlation analysis showed that serum sFas and 8-OHdG levels in AD patients were negatively correlated with Bifidobacterium and Lactobacillus bacteria abundance(P<0.05),and positively correlated with Staphylococcus bacteria abun-dance(P<0.05).ROC curve analysis results showed that the area under the curve(AUC)of serum sFas and 8-OHdG combined to predict severe AD was 0.870,which was larger than the AUC predicted by serum sFas alone(Z=2.011,P=0.044),and there was no statistical significance when compared with the AUC predicted by serum 8-OHdG alone(Z=1.878,P=0.060).Conclusion Serum sFas and 8-OHdG levels in AD patients are relatively high,and they are correlated with the abundance of intestinal flora abundance,which has certain predictive value for its severity.关键词
阿尔茨海默病/肠道菌群/可溶性凋亡相关因子/8-羟基脱氧鸟苷/预测价值Key words
Alzheimer's disease/intestinal flora/soluble apoptosis related factor/8-hydroxydeox-yguanosine/predictive value分类
医药卫生引用本文复制引用
王鹏飞,李艳雪,王禹,孙安龙..肠道菌群丰度及血清sFas、8-OHdG对重度阿尔茨海默病的预测价值[J].检验医学与临床,2025,22(8):1014-1018,1023,6.基金项目
河北省医学科学研究课题计划项目(20232047). （20232047）
