检验医学与临床2025，Vol.22Issue(8)：1116-1120,1126,6.DOI:10.3969/j.issn.1672-9455.2025.08.021
乳腺癌组织miR-216b-5p、miR-589-5p与肿瘤恶性增殖基因表达和预后的关系
Relationship between miR-216b-5p,miR-589-5p and tumor malignant proliferation gene expression and prognosis in breast cancer tissues
摘要
Abstract
Objective To investigate the relationship between miR-216b-5p,miR-589-5p and tumor malig-nant proliferation gene expression and prognosis in breast cancer tissues.Methods A total of 490 breast canc-er patients who were admitted to Nanchong Central Hospital from August 2017 to August 2020 were selected as study objects.MiR-216b-5p,miR-589-5p and malignant proliferating genes[Yes associated protein 1(YAP1),PIwiL-like protein 2(Piwil2)and zeste gene potentiator homology 2(EZH2)]levles in cancer tissue and paracancer tissue were detected by real-time fluorescence quantitative polymerase chain reaction.Patients were followed up for 3 years,and they were divided into survival group and death group according to overall survival.Pearson correlation was used to analyze the correlation between the levels of miR-216b-5p,miR-589-5p and the levels of malignant proliferation genes in breast cancer tissues.The 3 years survival of breast cancer patients was analyzed by Log-Rankχ2 and Kaplan-Meier survival curves.Multivariate Cox regression was used to analyze the influencing factors of death in breast cancer patients.Results MiR-216b-5p and miR-589-5p in breast cancer tissues were lower than those in paracancer tissues,while the messenger RNA(mRNA)levels of YAP1,Piwil2 and EZH2 were higher than those in paracancer tissues,with statistical significance(P<0.05).The proportions of patients with lymph node metastasis,TNM stage Ⅲ,miR-216b-5p low expression and miR-589-5p low expression in death group were higher than those in survival group,with statistical signifi-cance(P<0.05).Kaplan-Meier survival curve analysis results show that the survival curves of patients with miR-216b-5p low expression,miR-589-5p low expression,lymph node metastasis and TNM stage Ⅲ were low-er than those of patients with miR-216b-5p high expression,miR-589-5p high expression,no lymph node me-tastasis and TNM stageⅠ+Ⅱ respectively(P<0.05).Multivariate Cox regression analysis showed that lymph node metastasis,TNM stageⅢ,miR-216b-5p low expression and miR-589-5p low expression were risk factors for death of breast cancer patients(P<0.05).Conclusion Compared with paracancer tissue,miR-216b-5p and miR-589-5p in breast cancer tissues are decreased,which are associated with high expression of malignant proliferation genes,increased TNM staging,lymph node metastasis.关键词
乳腺癌/miR-216b-5p/miR-589-5p/恶性增殖基因/病理特征/预后Key words
breast cancer/microRNA-216b-5p/microRNA-589-5p/malignant proliferation gene/pathological feature/prognosis分类
医药卫生引用本文复制引用
何英,石先伟,张雪琳,刘臻臻,朱克鹏,尹均明,杜果城..乳腺癌组织miR-216b-5p、miR-589-5p与肿瘤恶性增殖基因表达和预后的关系[J].检验医学与临床,2025,22(8):1116-1120,1126,6.基金项目
四川省南充市2022年市校科技战略合作专项(22SXQT0195). （22SXQT0195）
