检验医学与临床2025，Vol.22Issue(8)：1121-1126,6.DOI:10.3969/j.issn.1672-9455.2025.08.022
血清lncRNA RPPH1、miR-145-3p对肺炎支原体感染患儿病情严重程度及预后的预测价值
Predictive value of serum lncRNA RPPH1 and miR-145-3p for the severity and prognosis of Mycoplasma pneumoniae infection in children
摘要
Abstract
Objective To explore the predictive value of serum lncRNA RPPH1 and miR-145-3p for the se-verity and prognosis of Mycoplasma pneumoniae(MP)infection in children.Methods A total of 124 children with MP infection who received treatment in the hospital from June 2020 to May 2023 were selected as the MP infection group,and 124 healthy children who underwent physical examination in the hospital during the same period were selected as the control group.According to the CURB-65 scoring system,the severity of MP-infec-ted children was assessed and divided into severe group and mild group.The patients were followed up for 6 months and divided into good prognosis group and poor prognosis group.The expression levels of serum ln-cRNA RPPH1 and miR-145-3p were detected by real-time fluorescence quantification and polymerase chain re-action.The correlation between the expression level of serum lncRNA RPPH1 and miR-145-3p in MP infected children was analyzed by Pearson correlation.Receiver operating characteristic(ROC)curve was drawn to an-alyze the predictive efficacy of serum lncRNA RPPH1 and miR-145-3p in severe MP infection and the predic-tive value of poor prognosis in children with MP infection.Multivariate Logistic regression was used to analyze the influencing factors of poor prognosis in children with MP infection.Results The expression level of serum lncRNA RPPH1 in MP infection group was lower than that in control group,and the expression level of serum miR-145-3p in MP infection group was higher than that in control group,with statistical significance(P<0.05).Fifty-nine children were included in the severe group and 65 children were included in the mild group.The expression level of lncRNA RPPH1 in severe group was lower than that in mild group,and the expression level of miR-145-3p in severe group was higher than that in mild group,with statistical significance(P<0.05).lncRNA RPPH1 and miR-145-3p were predicted to have binding sites.Pearson correlation analysis showed that the expression level of serum lncRNA RPPH1 was negatively correlated with the expression level of miR-145-3p in children with MP infection(r=-0.663,P<0.05).ROC curve analysis results showed that the area under the curve(AUC)of serum lncRNA RPPH1 and miR-145-3p combined to predict severe MP in-fection was 0.898,which was larger than the AUC predicted by serum lncRNA RPPH1 and miR-145-3p alone(Z=2.065,2.106;P=0.039,0.035).According to the follow-up results,76 children were included in the good prognosis group and 48 children were included in the poor prognosis group.The expression level of ser-um lncRNA RPPH1 in the poor prognosis group was lower than that in the good prognosis group,and the ex-pression level of serum miR-145-3p in the poor prognosis group was higher than that in the good prognosis group,with statistical significance(P<0.05).ROC curve analysis results showed that the AUC of serum lncRNA RPPH1 and miR-145-3p combined in predicting poor prognosis of MP-infected children was 0.927,which was larger than the AUC of serum lncRNA RPPH1 and miR-145-3p alone(Z=2.590,2.385,2.30;P=0.010,0.017).Multiva-riate Logistic regression analysis showed that serum lncRNA RPPH1<0.836 and miR-145-3p≥1.224 were risk fac-tors for poor prognosis in children with MP infection(P<0.05).Conclusion The serum lncRNA RPPH1 level in children with MP infection is greatly reduced,while the miR-145-3p level is greatly increased.The combination of the two has a better diagnostic effect on the severity of MP infection and has good prognostic evaluation value.关键词
长链非编码RNA RPPH1/微小RNA-145-3p/儿童/肺炎支原体/病情/预后Key words
long non-coding RNA RPPH1/microRNA-145-3p/children/mycoplasma pneumoniae/disease condition/prognosis分类
临床医学引用本文复制引用
马亚波,高珊,董艳菊..血清lncRNA RPPH1、miR-145-3p对肺炎支原体感染患儿病情严重程度及预后的预测价值[J].检验医学与临床,2025,22(8):1121-1126,6.基金项目
河北省保定市科技计划项目(2041ZF219). （2041ZF219）
