兰州大学学报（医学版）2025，Vol.51Issue(3)：14-20,7.DOI:10.13885/j.issn.1000-2812.2025.03.003
放射治疗对新型小鼠坐骨神经癌痛模型行为的影响
Effect of radiotherapy on neuropathy in a new sciatic nerve cancer pain model in mice
摘要
Abstract
Objective To investigate the effects of different intensities of radiotherapy on neuropathic pain in a new sciatic nerve cancer pain model in mice.Methods Twenty-four BALB/c female mice weighing 18-20 g were selected,and all were randomly divided into four groups using the random number table method:a con-trol group(Group Ctrl),a 6-gray radiotherapy group(Group 6 Gy),a 10-gray radiotherapy group(Group 10 Gy),and a 15-gray radiotherapy group(Group 15 Gy).The basal body weights,volumes of the left thighs,and bases of the left paws'mechanical withdrawal threshold(MWT)were recorded for each group.Colon cancer cells CT26 were inoculated near the sciatic nerve of each mouse,and data were recorded on consecutive days.On day 9,no radiotherapy was given to Group Ctrl.Radiotherapy of Group 6 Gy intensity was given to Group 6 Gy,10 Gy intensity to Group 10 Gy,and Group 15 Gy intensity to Group 15 Gy,and the dates were recorded on consecutive days.Results Compared with the Group Ctrl,the tumour volume growth rate in the Group 6 Gy was significantly decreased(P<0.01),the date of low peak and abnormal increase in MWT significantly delayed,and the date of appearance of paralysis of the affected limb significantly delayed(P<0.01);compared with Group 6 Gy,the tumour volume growth rate in Group 10 Gy was significantly decreased(P<0.01),the date of low peak and abnormal increase in MWT significantly delayed,the date of the appear-ance of affected limb paralysis delayed(P<0.05);compared with Group 10 Gy,the growth rate of tumour volume in Group 15 Gy was significantly decreased(P<0.01),MWT was not significantly reduced after treat-ment,no obvious low peak was observed,and no abnormal increase in MWT observed,and only one mouse was found to have developed limb paralysis on the 23rd day after treatment,and the date of the appearance of limb paralysis was not significantly different(P>0.05).Conclusion Results from pain behavioural studies sug-gest that the new sciatic nerve cancer pain model in mice has been successfully established and that 10 Gy is the preferred radiotherapy radiation intensity.关键词
神经病理性疼痛/癌痛模型/放射治疗/坐骨神经Key words
neuropathic pain/cancer pain model/radiotherapy/sciatic nerve引用本文复制引用
桂铭鸿,刘伟,王郭洁颖,牛永慧,刘璐,王迎斌..放射治疗对新型小鼠坐骨神经癌痛模型行为的影响[J].兰州大学学报（医学版）,2025,51(3):14-20,7.基金项目
甘肃省自然科学基金资助项目(22JR5RA980) （22JR5RA980）
兰州大学第二医院"萃英科技创新"计划资助项目(CY2023-BJ-11) （CY2023-BJ-11）
