兰州大学学报（医学版）2025，Vol.51Issue(3)：47-58,80,13.DOI:10.13885/j.issn.1000-2812.2025.03.007
胃癌m7G相关微小RNA预后模型的构建与验证
Construction and validation of a prognostic model of m7G-associated microRNA in gastric cancer
摘要
Abstract
Objective To investigate the predictive value of N-7 methylguanosine(m7 G)-related microRNA(miRNA)in gastric cancer prognosis models.Methods A m7 G-related miRNA prognostic model for gastric cancer was established,and the risk score computed based on this model.Patients with gastric cancer were classified into high-risk and low-risk groups,and their overall survival times were compared.Differences in tumor immune escape and sensitivity to immunotherapy drugs between high-risk and low-risk groups were further analyzed,and the correlation between tumor mutation burden and immunotherapy response was inves-tigated.Results A prognostic model consisting of 10 miRNA associated with m7 G was constructed.The overall survival of patients in the low-risk group was significantly longer compared to that of the high-risk group.Regression analysis indicated that the risk score could serve as an independent prognostic factor(risk score=1.009,hazard ratio=1.003-1.015,P<0.05).Based on the risk score and clinicopathological factors,the area under the subject operation characteristic curve for predicting 1-year,3-year,and 5-year survival rates of gastric cancer patients was 0.753,0.768,and 0.749,respectively.Significant differences were observed in tu-mor mutation burden and the effectiveness of immunotherapy between the two groups.Real-time quantitative PCR results revealed that hsa-mir-200c,hsa-mir-942,and hsa-mir-3138 were significantly downregulated in tumor tissues.Conclusion The m7 G-related miRNA prognostic risk model serves as a potential tool for sur-vival prediction and immunotherapy stratification in gastric cancer patients.关键词
N7-甲基鸟苷/甲基化/微小核糖核酸/预后/肿瘤突变负荷/胃癌Key words
m7 G/methylation/microRNA/prognosis/tumor mutation burden/gastric cancer分类
临床医学引用本文复制引用
余春燕,刘涛,李亚西,杨晓晓,黄翌楚,李桐桐,闵光涛,姜雷..胃癌m7G相关微小RNA预后模型的构建与验证[J].兰州大学学报（医学版）,2025,51(3):47-58,80,13.基金项目
国家自然科学基金资助项目(82060527) （82060527）
兰州市科技发展指导性计划资助项目(2020-ZD-66) （2020-ZD-66）
