临床口腔医学杂志2025，Vol.41Issue(4)：205-209,5.DOI:10.3969/j.issn.1003-1634.2025.04.004
3D打印纳米多肽负载人牙周膜干细胞支架的体外成骨分化研究
In vitro osteogenic differentiation study of 3D printed nano peptide loaded human periodontal ligament stem cell scaffold
摘要
Abstract
Objective:To prepare self-assembling peptide nanofiber scaffolds(SAPNS)/human periodontal ligament stem cells(hPDLSCs)by 3D bioprinting technology,then to investigate osteogenic differentiation in vitro.Methods:Firstly,SAPNS was constructed using RADA16-I,ALK,and WP9QY peptides.Then SAPNS scaffolds were prepared using a 3D printer,in addition,3D printing was used to prepare polylactic acid scaffolds.The third-generation hPDLSCs was took and i-dentified surface markers using flow cytometry.hPDLSC and SAPNS were comixed to form a"bio ink"for printing SAPNS/hPDLSCs cell scaffolds,in addition,polylactic acid/hPDLSCs cell scaffolds was also printed.The experiment randomly divid-ed hPDLSCs into control group,polylactic acid group,and SAPNS group.Alizarin red and alkaline phosphatase(ALP)stai-ning were performed on the 7th and 14th day of culture,respectively.Real time fluorescence quantitative PCR(qRT-PCR)was used to detect mRNA expressions of osteogenic differentiation related genes,including Runt related transcription factor 2(RUNX2),ALP,type Ⅰ collagen(COL1),and osteocalcin(OCN).Results:Compared with control group,the formation of mineralized nodules and mineral content of osteoblasts in polylactic acid group increased significantly on the 7th and 14th days,with SAPNS group having the highest amount,mRNA expressions of RUNX2,ALP,COL1,and OCN also significantly increased,with the highest expressions in SAPNS group(P＜0.05).Conclusion:3D printing SAPNS as scaffold material has better osteogenic differentiation performance in hPDLSCs compared to conventional polylactic acid.关键词
3D生物打印/自组装纳米多肽水凝胶/人牙周膜干细胞/聚乳酸/成骨Key words
3D bioprinting/Self-assembling peptide nanofiber scaffolds/Human periodontal ligament stem cells/Polylactic acid/Osteogenesis分类
生物工程引用本文复制引用
吐尔洪阿依·塔西甫拉提,茹菲亚·祖拉提,裴培,姬晓炜..3D打印纳米多肽负载人牙周膜干细胞支架的体外成骨分化研究[J].临床口腔医学杂志,2025,41(4):205-209,5.基金项目
新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY-202450) （WJWY-202450）
