现代畜牧兽医Issue(3)：16-20,5.DOI:10.20154/j.cnki.issn1672-9692.2025.03.004
致奶牛乳房炎大肠埃希菌的分离鉴定及耐药分析
Isolation,identification and drug resistance analysis of Escherichia coli that causes mastitis in dairy cows
摘要
Abstract
The experiment aimed to investigate the antibiotic resistance and pathogenicity of Escherichia coli causing bovine mastitis in the Shihezi region.The experiment involved bacterial isolation and purification from milk samples of dairy cows with mastitis in the surrounding areas of Shihezi.The isolated strains underwent biochemical identification,Gram staining and microscopic examination,16S rDNA identification,as well as pathogenicity tests and antimicrobial susceptibility testing.The results showed that a suspected Escherichia coli strain was isolated,exhibiting an oval or rod-shaped morphology with Gram-negative staining(red coloration).Biochemical identification yielded a characteristic profile code of A070,and 16S rDNA sequencing confirmed it as Escherichia coli.Antimicrobial susceptibility testing revealed resistance to β-lactams,aminoglycosides,and quinolones.Pathogenicity tests demonstrated that the isolate caused mortality in mice,with deceased subjects exhibiting hepatomegaly and congestion spots in the liver,spleen,and heart.This study successfully isolated a pathogenic Escherichia coli strain from raw milk sample showing partial antibiotic resistance.The findings provide a reference for clinical treatment strategies against bovine mastitis in dairy farms.关键词
大肠埃希菌/分离鉴定/药敏试验/致病性试验Key words
Escherichia coli/Isolation and identification/Drug susceptibility testing/Pathogenicity testing分类
农业科技引用本文复制引用
丁颖,张伟,魏铭清,刘非凡,张倩,周霞,王震,张辉..致奶牛乳房炎大肠埃希菌的分离鉴定及耐药分析[J].现代畜牧兽医,2025,(3):16-20,5.基金项目
江苏省人兽共患病学重点实验室资助项目(R2308) （R2308）
新疆生产建设兵团科技计划项目(2024AB035) （2024AB035）
