现代畜牧兽医Issue(3)：21-25,5.DOI:10.20154/j.cnki.issn1672-9692.2025.03.005
1株鸽源唾液乳杆菌的分离鉴定及生物学特性分析
Isolation,identification,and biological characteristics analysis of a pigeon-derived Lactobacillus salivarius strain
摘要
Abstract
The experiment aimed to screen Lactobacillus strains specifically for meat pigeons.Pharyngeal swabs collected from meat pigeons in the poultry house were subjected to pathogen isolation and identification,morphological observation,biochemical characterization,and 16S rDNA sequence analysis.Additionally,the isolated strains were studied for acid tolerance,bile salt tolerance,thermal tolerance,and antimicrobial activity.The results showed that the isolate was identified as Lactobacillus salivarius through morphological observation,biochemical characterization,and 16S rDNA sequence analysis,and was designated as C-1-3-1.The survival rate of isolate C-1-3-1 exceeded 70%at 40℃and 45℃,and it exhibited limited survival in MRS medium containing 0.20%porcine bile salt.At pH 4,the strain's survival rate reached 63.0%,while at pH 5,the survival rate exceeded 90%.The isolate inhibited the growth of Escherichia coli and Staphylococcus aureus,with enhanced antimicrobial effects when combined with Patrinia scabiosifolia extract.The study demonstrated that isolate C-1-3-1 possesses strong thermal tolerance,acid tolerance,bile salt tolerance,and promising probiotic properties,making it a potential candidate for probiotic development.关键词
唾液乳杆菌/分离鉴定/败酱草提取液/抑菌性能Key words
Lactobacillus salivarius/Isolation and identification/Patrinia scabiosifolia extract/Antibacterial performance分类
农业科技引用本文复制引用
党乙凡,牛梅婷,李涛,张蒙林,陈荣..1株鸽源唾液乳杆菌的分离鉴定及生物学特性分析[J].现代畜牧兽医,2025,(3):21-25,5.基金项目
兵团指导性科技计划项目(2022ZD111、TDZKKY-202203) （2022ZD111、TDZKKY-202203）
