种业导刊Issue(2)：17-23,7.DOI:10.3969/j.issn.1003-4749.2025.02.004
黄瓜持绿蛋白与候选分泌效应蛋白互作分析
Analysis of Interaction between Cucumber Stay Green Protein and Candidate Secreted Effector Protein
摘要
Abstract
The yeast two-hybrid technology was used to screen candidate secreted effector proteins(CSEP)that interact with cucumber stay green protein(SGR).The interaction was further validated by GST-pull down and co-immunoprecipitation techniques.The results showed that the cucumber CsSGR and CsCSEP genes were successfully cloned.The yeast two-hybrid assay revealed that CsSGR protein interacted with CsCSEP30,CsCSEP47 and CsCSEP48 proteins.Both GST-pull down and co-immunoprecipitation assays confirmed the interaction between CsSGR protein and CsCSEP30,CsCSEP47 and CsCSEP48 proteins.In conclusion,CsSGR protein interacts with CsCSEP30,CsCSEP47 and CsCSEP48 proteins,CsSGR may help to delay leaf senescence and enhance disease resistance.关键词
黄瓜/持绿蛋白/候选分泌效应蛋白/蛋白互作/酵母双杂交/免疫共沉淀Key words
Cucumber/Stay green protein/Candidate secreted effector protein/Protein interaction/Yeast two-hybrid/Co-immunoprecipitation分类
园艺学与植物营养学引用本文复制引用
陈蒙,邹杭宇,刘海峰,李正焕..黄瓜持绿蛋白与候选分泌效应蛋白互作分析[J].种业导刊,2025,(2):17-23,7.基金项目
韩国农业发展局(RDA)项目(PJ01596502) （RDA）
韩国全北国立大学BK21四大项目 （）
延边大学创业项目 （）
