南通大学学报（自然科学版）2025，Vol.24Issue(1)：1-9,9.DOI:10.12194/j.ntu.20240725001
酶响应型荧光探针在耐药菌检测中的应用
Application of enzyme-responsive fluorescent probes in detecting drug-resistant bacteria
摘要
Abstract
With the overuse of clinical antibiotics,the types and varieties of drug-resistant bacteria are continually in-creasing.The timely and accurate identification of bacterial strains is crucial for the prevention and control of drug-re-sistant bacteria and the development of antibiotics.Fluorescent probes for detecting key bacterial enzyme metabolites,as a novel detection tool,can overcome the limitations of traditional detection methods and show great potential and advantages in detecting drug-resistant bacteria.This paper focuses on fluorescent probes for detecting key enzyme metabolites of clinically common drug-resistant bacteria,summarizing the applications and principles of enzyme-re-sponsive fluorescent probe technology in detecting drug-resistant bacteria in recent years.The study reviews the rela-tionship between bacterial resistance mechanisms and enzyme metabolites,highlighting the differences in enzyme metabolism between resistant and sensitive bacteria.Subsequently,based on the analysis of characteristic enzyme metabolites of various drug-resistant bacteria,the working principle of fluorescent probes for detecting drug-resistant bacteria is elucidated.Additionally,the study explores possible directions for optimizing fluorescent probes,providing new insights for faster and more accurate diagnosis and treatment of drug-resistant bacteria.关键词
耐药菌/细菌代谢物/荧光探针/β-内酰胺酶/硝基还原酶Key words
drug-resistant bacteria/bacterial metabolites/fluorescent probes/β-lactamase/nitroreductase分类
临床医学引用本文复制引用
管佳赟,张靖怡,王励,于滟扬,王琦,秦玉岭,吴丽..酶响应型荧光探针在耐药菌检测中的应用[J].南通大学学报（自然科学版）,2025,24(1):1-9,9.基金项目
国家自然科学基金面上基金项目(32171452,82373456) （32171452,82373456）
国家自然科学基金青年科学基金项目(62005132) （62005132）
