南通大学学报（自然科学版）2025，Vol.24Issue(1)：66-73,84,9.DOI:10.12194/j.ntu.20240108001
陆地棉RVT_3基因家族的全基因组分析
Genome-wide analysis of RVT_3 gene family in upland cotton
摘要
Abstract
Soil salinization induces salt stress,which restricts plant growth and development,presenting significant ob-stacles to agricultural production and constraining productivity gains.To shift from managing saline-alkali lands to se-lecting salt-tolerant crops adapted to such conditions,identifying effective salt-tolerance genes in crops is essential.The RVT_3 gene family,part of the retrotransposon group,features domains widely distributed across plant species.Prior salt-stress transcriptome analysis of upland cotton revealed that multiple genes within this family are associated with salt tolerance.In this study,bioinformatics analysis of the RVT_3 family was performed using the PFam database,identifying 173 genes across the upland cotton genome.Analyses of phylogeny,gene structure,chromosome localiza-tion,and gene duplication were conducted,alongside an examination of cis-acting elements within these genes.Chro-mosome mapping revealed numerous fragment duplication events among RVT_3 genes.Phylogenetic,gene structure,and conserved motif analyses indicated that genes within the same subfamily exhibit comparable structures and motifs.Cis-acting element analysis suggested potential involvement of this gene family in responses to diverse biotic and abiotic stresses.The RVT_3 proteins were classified into three distinct subfamilies,with structural similarities among genes within each subgroup supporting subfamily differentiation.Of the 173 genes,146 are distributed across the 26 chromosomes of upland cotton.RT-qPCR data further confirmed the responsiveness of this gene family to salt stress in upland cotton.These findings offer insights into the evolutionary relationships and functional roles of the RVT_3 gene family,establishing a basis for deeper investigation into cotton's salt-tolerance mechanisms and supporting molecular breeding efforts for enhanced salt tolerance.关键词
陆地棉/RVT_3/基因家族/盐胁迫/逆转录转座子Key words
upland cotton/RVT_3/gene family/salt stress/retrotransposons分类
农业科技引用本文复制引用
谷海静,孙康泰,唐峻峰,吴赟浩,高昕,陈俊宇,李烨雄,杨一轩,汪保华..陆地棉RVT_3基因家族的全基因组分析[J].南通大学学报（自然科学版）,2025,24(1):66-73,84,9.基金项目
国家重点研发计划项目(2021YFE0101200) （2021YFE0101200）
江苏省中学生科技创新后备人才培养计划项目(苏青科函[2023]7号) （苏青科函[2023]7号）
