农业环境科学学报2025，Vol.44Issue(3)：696-707,12.DOI:10.11654/jaes.2025-0141
锰矿区周边农田土壤HMs-PAHs共存污染特征及土壤微生物的响应
Characterization of heavy metal-polycyclic aromatic hydrocarbon co-contamination in agricultural soils surrounding mining areas and the response of soil microorganisms
摘要
Abstract
This study investigated the co-contamination characteristics of heavy metals(HMs)and polycyclic aromatic hydrocarbons(PAHs)in the dryland and paddy soils surrounding certain manganese mining areas in Huayuan County,Hunan Province.The contents of HMs and PAHs in the soils of drylands and paddy fields were analyzed by inductively coupled plasma mass spectrometry(ICP-MS)and gas chromatography-mass spectrometry(GC-MS),respectively.The potential ecological risk assessment method and contamination factor(CF)approach were employed to evaluate the risk assessment about the co-contamination of HMs and PAHs in the dryland and paddy soils.Additionally,microbial community structure characteristics and resistance mechanisms in soils with different pollution degrees of HMs and PAHs were investigated through high-throughput sequencing of 16S rDNA and real-time fluorescence quantitative PCR analysis.The results indicate that the contents of cadmium(Cd),arsenic(As),and PAHs in dryland soils are 10.4,1.43 folds,and 1.8 folds of the risk screening values for agricultural soil pollution,respectively.In paddy soils,the contents of Cd and PAHs are 3.9 folds and 1.1 folds of the risk screening values for agricultural soil pollution,respectively.Moreover,the bioavailability of Cd in dryland soils was significantly higher than that in paddy soils.Based on the comprehensive pollution index,both dryland and paddy soils at the sampling sites can be categorized into heavy,moderate,and light pollution zones,with the risk order of contamination factors being Cd>As>Pb>PAHs.Significant differences in microbial community structures were observed among soils with different pollution degrees of HMs and PAHs,where the dominant genera in heavily polluted,moderately polluted,and lightly polluted soils were Acidobacteria Gp6,Acidobacteria Gp4,and Bacillus,respectively.In dryland soils,the predominant genera included Acidobacteria and Sphingomonas,while Bacillus was the dominant genus in paddy soils.In dryland,Cd is the main influencing factor of the dominant bacterial community,while in paddy fields,PAHs take on this role.The pollution level is significantly positively correlated with the abundance of HMs tolerance genes and PAHs degradation genes in functional strains.The relative abundance of Sphingomonas is significantly positively related to the co-contamination levels of HMs and PAHs,suggesting its potential as a biomarker for combined contamination in the area.关键词
锰矿区/重金属/多环芳烃/共存污染/微生物Key words
mining area/heavy metals/polycyclic aromatic hydrocarbons/co-contamination/microbiology分类
资源环境引用本文复制引用
彭冠维,易盛炜,张祺明,刘杰,甘杰,黄钟霆,石雪芳,吴玉俊,李峰..锰矿区周边农田土壤HMs-PAHs共存污染特征及土壤微生物的响应[J].农业环境科学学报,2025,44(3):696-707,12.基金项目
国家自然科学基金项目(22176161) （22176161）
国家自然科学基金区域创新发展联合基金重点支持项目(U21A20293) （U21A20293）
湖南省教育厅资助科研项目(24A0127) （24A0127）
国家环境保护重金属污染监测重点实验室项目(KLMHM202405) （KLMHM202405）
国家环境保护重金属污染监测重点实验室开放基金项目(SKLMHM202303) National Natural Science Foundation of China(22176161) （SKLMHM202303）
National Natural Science Foundation of China-Hunan Joint Fund(U21A20293) （U21A20293）
Research Foundation of Education Bureau of Hunan Province(24A0127) （24A0127）
State Key Laboratory of Heavy Metal Pollution Monitoring for Environmental Protection(KLMHM202405) （KLMHM202405）
Open Fund Program of State Key Laboratory of Heavy Metal Pollution Monitoring for Environmental Protection(SKLMHM202303) （SKLMHM202303）
