农业环境科学学报2025，Vol.44Issue(3)：741-749,9.DOI:10.11654/jaes.2024-1127
高分辨质谱结合分子网络技术在鱼体恩诺沙星及其转化产物分析中的应用
Application of high-resolution mass spectrometer combined with molecular networking in the analysis of enrofloxacin and its transformation product in fish
摘要
Abstract
This study aimed to identify the transformation products of enrofloxacin,a widely used antibiotic in aquaculture,within complex biological environments.To achieve this,a molecular network was constructed to confirm the structures of molecular clusters and related primary compounds.The analysis integrated mass spectrum fragmentation patterns of enrofloxacin with high-resolution primary and secondary mass spectrometry data.The primary objective was to identify enrofloxacin transformation products across various tilapia tissues and to explore the active sites of enrofloxacin biotransformation.The results identified ten potential transformation products of enrofloxacin in tilapia tissues using molecular network technology,with ciprofloxacin detected across all tissues.The quinolone and piperazine rings of enrofloxacin were pinpointed as potential conversion sites,indicating that the biotransformation processes in tilapia primarily involve reactions between the quinolone ring(4 items)and piperazine ring(6 items).This study demonstrates the effectiveness of combining high-resolution mass spectrometry with molecular networking to identify enrofloxacin transformation products,offering valuable insights for detecting and tracking emerging contaminants of antibiotic in complex environments.关键词
分子网络/转化产物/恩诺沙星/罗非鱼/产物鉴定Key words
molecular network/transformation product/enrofloxacin/tilapia/product identification分类
资源环境引用本文复制引用
朱磊,方龙香,李丹丹,胡庚东,裘丽萍,孟顺龙,宋超..高分辨质谱结合分子网络技术在鱼体恩诺沙星及其转化产物分析中的应用[J].农业环境科学学报,2025,44(3):741-749,9.基金项目
中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2023JBFM02) （2023JBFM02）
无锡市科技发展资金项目(K20231029) Central Public-Interest Scientific Institution Basal Research Fund,the Freshwater Fisheries Research Center,CAFS(2023JBFM02) （K20231029）
Science and Technology Development Fund of Wuxi,China(K20231029) （K20231029）
