农业环境科学学报2025，Vol.44Issue(3)：815-828,14.DOI:10.11654/jaes.2024-1164
自然土壤中6:2氯代多氟烷基醚磺酸对蚯蚓的毒性效应
Toxicological effects of 6:2 Cl-PFESA on earthworms in natural soil
摘要
Abstract
To investigate the toxicity and molecular mechanisms of 6:2 chlorinated polyfluoroalkyl ether sulfonic acid(6:2 Cl-PFESA)on earthworms,the 28-day toxic effects of 6:2 Cl-PFESA(0.1,1,10 mg·kg-1)on earthworms were investigated using the natural soil method in this study.Moreover,enzymatic assays,amplicon sequencing,and metabolomics techniques were used to explore the underlying molecular mechanisms of toxicity.Results showed that,the bioaccumulation factors of 6:2 Cl-PFESA in earthworms ranged from 2.43 to 3.81 after 28 days of exposure,and significant reductions in survival rates were observed only at the highest concentration(10 mg·kg-1).At the sub-individual level,exposure to 6:2 Cl-PFESA at all tested concentrations induced oxidative stress in earthworms,with catalase(CAT)and peroxidase(POD)showing higher sensitivity to 6:2 Cl-PFESA than superoxide dismutase(SOD).Furthermore,exposure to 1 mg·kg-1 and 10 mg·kg-1 resulted in significant lipid peroxidation damage and neurotoxicity.Microbial diversity analysis showed that exposure to 10 mg·kg-1 6:2 Cl-PFESA significantly reduced the relative abundance of probiotic bacteria(Lactobacillus),while increased the relative abundance of pathogenic bacteria(Verrucomicrobiota).Metabolomics analysis showed that exposure to 10 mg·kg-1 6:2 Cl-PFESA mainly upregulated key metabolites such as amino acids/peptides and their analogues,carbohydrates and their conjugates,disrupting key metabolic pathways such as glycerophospholipid metabolism,ultimately impairing normal physiological functions of earthworms.Overall,this study indicated that after 28 days of exposure,6:2 Cl-PFESA can bioaccumulate in earthworms.Even exposure to low concentration of 6:2 Cl-PFESA in soil(0.1 mg·kg-1)can induce oxidative stress in earthworms.Higher concentrations of 6:2 Cl-PFESA(1 mg·kg-1 and 10 mg·kg-1)lead to significant lipid peroxidation damage and neurotoxicity in earthworms.Exposure to 10 mg·kg-1 6:2 Cl-PFESA causes dysbacteriosis and metabolic dysfunction in earthworm.关键词
6:2氯代多氟烷基醚磺酸/自然土壤/蚯蚓/毒性/酶学/微生物多样性/代谢组学Key words
6:2 chlorinated polyfluoroalkyl ether sulfonic acid/natural soil/earthworm/toxicity/enzymatic assays/microbial diversity/metabolomics分类
资源环境引用本文复制引用
秦成,赵美静,路畅,王俊杰,赵丽霞,李晓晶..自然土壤中6:2氯代多氟烷基醚磺酸对蚯蚓的毒性效应[J].农业环境科学学报,2025,44(3):815-828,14.基金项目
国家重点研发计划项目(2022YFC3703200,2024YFD1701100) （2022YFC3703200,2024YFD1701100）
国家自然科学基金项目(42177034,41601342) （42177034,41601342）
中国农业科学院科技创新工程项目(CAAS-CSGLCA-202302) National Key Research and Development Program of China(2022YFC3703200,2024YFD1701100) （CAAS-CSGLCA-202302）
National Natural Science Foundation of China(42177034,41601342) （42177034,41601342）
Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSGLCA-202302) （CAAS-CSGLCA-202302）
