农业环境科学学报2025，Vol.44Issue(3)：839-848,10.DOI:10.11654/jaes.2024-1131
太平洋牡蛎溶菌酶基因对4种磺胺类抗生素的表达响应
Expression response of the lysozyme gene in Pacific oysters(Crassostrea gigas)to four sulfonamide antibiotics
摘要
Abstract
To investigate the expression response of the lysozyme gene(LZM gene)in Pacific oysters(Crassostrea gigas)to four sulfonamide antibiotics,this study conducted exposure experiments using these antibiotics.Based on environmental survey concentrations of sulfonamides in marine systems,exposure doses were set at 5 μg·L-1 and 500 μg·L-1.Acute(48 h)and sub-chronic(14 d)antibiotic exposures were performed,followed by Vibrio parahaemolyticus challenge assays.Hepatopancreas and gill tissues were sampled for LZM gene expression analysis.The four antibiotics exhibited marked differences in their effects on LZM gene expression and associated biotoxic effects in Crassostrea gigas.Sulfamonomethoxine(SMT)and sulfadoxine(SDX)demonstrated significant inhibitory activity on LZM gene expression in both gill tissues(SMT and SDX inhibition rate was approximately 69%and 72%,respectively)and hepatopancreas(SMT and SDX inhibition rate was approximately 70%and 74%,respectively)compared to control.In contrast,sulfamonomethoxine(SMM)demonstrated better biological immune adaptability,with its toxic effects markedly increasing under high-concentration,long-term exposure.After bacterial challenge experiments,SMT and SDX exposure did not reduce the oysters′ immune capacity against Vibrio compared to the control group.SMM and sulfamethoxazole(SMX)appeared to enhance the oysters′ immune response to Vibrio.Additionally,the immune response of oysters to Vibrio exhibited tissue-specific differences,with more pronounced LZM gene expression in the hepatopancreas compared to gill tissues.The study concluded that sulfonamide antibiotic exposure can alter the expression of the LZM gene in Pacific oysters.However,further research is required to elucidate the mechanisms underlying the bioeffects of sulfonamide antibiotics on oysters and determine the threshold for their impact on oyster immunity.关键词
磺胺类抗生素/太平洋牡蛎/溶菌酶/基因表达Key words
sulfonamide antibiotics/Crassostrea gigas/lysozyme/gene expression分类
资源环境引用本文复制引用
陈昊,李淑君,叶琳,冯晓龙,孙婧,苏博,吉成龙,由丽萍,马元庆..太平洋牡蛎溶菌酶基因对4种磺胺类抗生素的表达响应[J].农业环境科学学报,2025,44(3):839-848,10.基金项目
山东省重点研发计划课题(重大科技创新工程)(2022CXGC020416) Key Research and Development Program of Shandong Province,China(2022CXGC020416) （重大科技创新工程）
