农业生物技术学报2025，Vol.33Issue(4)：731-742,12.DOI:10.3969/j.issn.1674-7968.2025.04.003
马铃薯StDHN-2基因的克隆与表达分析
Cloning and Expression Analysis of the StDHN-2 Gene in Potato(Solanum tuberosum)
摘要
Abstract
Plant dehydrins(dehydrins,DHN)are a class of specific proteins expressed when plants are subjected to adversities such as drought and salinity.To investigate the function of potato(Solanum tuberosum)StDHN-2 gene in plant drought tolerance,in this study,the CDS of StDHN-2 gene was cloned from potato cultivar'Atlantic'plantlets,and the fusion protein expression vector was constructed by homologous recombination,the subcellular localization results of StDHN-2 protein showed that the protein might be located in the nucleus.Based on the bioinformatics analysis of StDHN-2 gene,it was found that the genome had a total length of 931 bp,including a coding region sequence of 474 bp,which encoded 157 amino acids;The molecular weight of StDHN-2 protein was 16 659.06 D,and the theoretical isoelectric point was 7.23.The analysis showed that StDHN-2 protein was a hydrophilic protein without transmembrane domain.The secondary structure of the StDHN-2 protein was mainly the random coil,and there was no β-turn structure.By constructing a phylogenetic tree,it was found that StDHN-2 protein had the closest evolutionary relationship with Solanum verrucosum;The cis-acting element analysis showed that the promoter region of StDHN-2 gene contained ABRE,DRE,P-box,LTR and other elements related to abiotic stress response;The expression patterns of StDHN-2 gene were analyzed under PEG,NaCl,and abscisic acid(ABA)stress treatments by using qRT-PCR analysis,it was found that the gene was up-regulated under all 3 kinds of stress treatments.The results of the study preliminarily indicated that the StDHN-2 gene could be a good candidate gene for drought tolerance in potato and involved in the biological process of drought tolerance in plants.This study provides basic information for analyzing the drought response mechanism of potato StDHN-2 gene.关键词
马铃薯/StDHN-2基因/表达分析/生物信息学分析/亚细胞定位Key words
Potato/StDHN-2 gene/Expression analysis/Bioinformatics analysis/Subcellular localization分类
农业科学引用本文复制引用
魏晶晶,马丽丽,张宁,司怀军..马铃薯StDHN-2基因的克隆与表达分析[J].农业生物技术学报,2025,33(4):731-742,12.基金项目
国家重点研发计划(2022YFD1602103) （2022YFD1602103）
甘肃省科技重大专项计划(23ZDNA006) （23ZDNA006）
