农业生物技术学报2025，Vol.33Issue(4)：755-770,16.DOI:10.3969/j.issn.1674-7968.2025.04.005
茶树醛脱氢酶(ALDH)超家族的鉴定与表达分析
Identification and Expression Analysis of the Aldehyde Dehydrogenases(ALDHs)Superfamily in Tea Plant(Camellia sinensis)
摘要
Abstract
Aldehyde dehydrogenases(ALDHs)play an important role in aldehyde stabilization under abiotic stress in plants.The members of ALDH superfamily in tea plant(Camellia sinensis)were identified by bioinformatics methods,and the biological information such as physicochemical properties,chromosome localization,gene structure,promoter cis-acting elements and phylogenetic relationship of the encoded proteins were analyzed.The expression patterns of the CsALDH superfamily members under drought,salt,and cold stresses ware analyzed by qRT-PCR.The results showed that a total of 27 ALDH superfamily members were identified in the C.sinensis genome,and phylogenetic analyses classified them into 10 families.Family members exhibited comparable motif composition and gene structure.The co-linearity analysis indicated that CsALDHs genes underwent gene duplication events in the C.sinensis genome,which experienced purifying selective pressures.The qRT-PCR results showed that 8,10 and 9 candidate CsALDHs genes were induced by PEG,NaCl and low temperature,respectively.In particular,CsALDH2B4 and CsALDH3H2 were significantly up-regulated.This study provides a theoretical foundation for further research on the functions of ALDH genes in tea plants.关键词
茶树/醛脱氢酶/全基因组鉴定/系统进化/非生物胁迫Key words
Camellia sinensis/Aldehyde dehydrogenases(ALDHs)/Genome-wide identification/Phylogeny/Abiotic stress分类
农业科学引用本文复制引用
刘丹丹,王雷刚,孙明慧,焦小雨,阮旭,吴琼,王文杰..茶树醛脱氢酶(ALDH)超家族的鉴定与表达分析[J].农业生物技术学报,2025,33(4):755-770,16.基金项目
国家自然科学基金联合基金(U23A20213) （U23A20213）
安徽省茶叶产业技术体系(AHCYJSTX-11) （AHCYJSTX-11）
安徽省农业科学院成果转化项目(2024YL041) （2024YL041）
