农业生物技术学报2025，Vol.33Issue(4)：771-781,11.DOI:10.3969/j.issn.1674-7968.2025.04.006
梅花GATA基因家族鉴定及PmGATA12在耐寒性调控中的作用分析
Identification of the Prunus mume GATA Gene Family and Analysis of the Role of PmGATA12 in Cold Tolerance Regulation
摘要
Abstract
Prunus mume,a traditional famous flower in China,holds significant ornamental value.GATA transcription factors play a crucial role in the regulation of plant growth and development as well as in the response to abiotic stress.In this study,a genome-wide identification of the GATA gene family in P.mume was conducted,and their expression patterns following cold treatment were systematically analyzed,with the functional characterization of PmGATA12 being comprehensively investigated.The results indicated that a total of 20 PmGATA genes were identified through whole-genome analysis,distribute across 7 chromosomes.Phylogenetic analysis revealed that PmGATA proteins could be divided into 3 subfamilies.Members of the same subfamily have similar conserved motifs and gene structures.Furthermore,by integrating transcriptome and qRT-PCR expression analysis,4 differentially expressed PmGATA genes(PmGATA6,PmGATA11,PmGATA12,and PmGATA 14)were identified.The genetic transformation of Arabidopsis thaliana with PmGATA12 significantly enhanced the cold tolerance of the transgenic lines.This study provides valuable gene resources for the cold-resistance breeding of P.mume and offers new insights into the molecular mechanisms underlying its cold tolerance.关键词
梅花/GATA/表达模式/耐寒Key words
Prunus mume/GATA/Expression patterns/Cold tolerance分类
园艺学与植物营养学引用本文复制引用
刘钊良,张军,金小军,金松恒,董彬..梅花GATA基因家族鉴定及PmGATA12在耐寒性调控中的作用分析[J].农业生物技术学报,2025,33(4):771-781,11.基金项目
浙江农林大学学校科研发展基金(2017FR016) （2017FR016）
