农业生物技术学报2025，Vol.33Issue(4)：782-791,10.DOI:10.3969/j.issn.1674-7968.2025.04.007
猪PLAG1基因过表达对3T3-L1前脂肪细胞增殖分化的影响
Effects of Overexpression of Porcine(Sus Scrofa)PLAG1 Gene on Proliferation and Differentiation of 3T3-L1 Preadipocytes
摘要
Abstract
Pleomorphic adenoma gene 1(PLAG1)plays a crucial role in regulating the growth and development of livestock and poultry.Our previous study identified that PLAG1 was associated with backfat thickness(BF)in pigs by genome-wide association study(GWAS),suggesting a potential regulatory effect on adipocytes.To further investigate the relationship between PLAG1 gene and adipocytes,3T3-L1 cells was used as a research model and PLAG1 conservation was analyzed.The effects of PLAG1 gene overexpression on cell proliferation and differentiation were examined using qRT-PCR,CCK-8 assays,flow cytometry,and Oil Red O staining.Results revealed high conservation of the PLAG1 gene,with sequence similarity among different species ranging from 80％to 92％.qRT-PCR analysis showed that,compared with the control group,the expression of proliferation-related genes in 3T3-L1 cells was extremely significantly increased following PLAG1 overexpression(P＜0.01).CCK-8 assays showed that PLAG1 overexpression extremely significantly enhanced cell viability(P＜0.01).Flow cytometry demonstrated a significant increase in the number of cells in the S phase following PLAG1 overexpression(P＜0.05).Furthermore,Oil Red O staining indicated that the expression of adipogenic differentiation-related genes was significantly decreased after PLAG1 overexpression(P＜0.05),accompanied by a extremely significant reduction in lipid droplet formation(P＜0.01).In summary,PLAG1 overexpression promoted the proliferation of 3T3-L1 cells while inhibiting their differentiation,indicating that PLAG1 gene might play a regulatory role in the proliferation and differentiation of adipocytes.This study provides a theoretical foundation for further investigation of the molecular mechanisms through which the PLAG1 gene regulates fat deposition in pigs.关键词
多形性腺瘤基因1(PLAG1)/猪/增殖分化/基因功能/3T3-L1前脂肪细胞Key words
Plemorphic adenoma gene 1(PLAG1)/Pigs/Proliferation and differentiation/Gene function/3T3-L1 preadipocytes分类
畜牧业引用本文复制引用
吴姿仪,李秀领,白利瑶,吴嘉浩,窦腾飞,李新建,韩雪蕾,乔瑞敏,王克君,杨峰..猪PLAG1基因过表达对3T3-L1前脂肪细胞增殖分化的影响[J].农业生物技术学报,2025,33(4):782-791,10.基金项目
国家重点研发计划(2021YFD1301201) （2021YFD1301201）
河南高等学校重点科研项目(23A230012) （23A230012）
河南省农业良种联合攻关项目(2022020101) （2022020101）
