农业生物技术学报2025，Vol.33Issue(4)：792-801,10.DOI:10.3969/j.issn.1674-7968.2025.04.008
一氧化氮合酶在奶牛临床型乳房炎乳腺组织中的表达、定位及功能分析
Expression,Localization and Functional Analysis of NOS in Mammary Gland Tissues of Dairy Cows(Bos taurus)with Clinical Mastitis
摘要
Abstract
Nitric oxide synthase(NOS),a crucial enzyme responsible for production of endogenous nitric oxide(NO)in body,has multiple effects such as anti-inflammatory or pro-inflammatory.To investigate the expression patterns and potential functions of NOS in dairy cows(Bos taurus)with healthy or clinical mastitis(CM),the mammary gland tissues were collected from dairy cows with healthy(control,Con)and CM in this study.The distribution and expression patterns of NOS were in the mammary gland tissues of the Con and CM groups using hematoxylin-eosin(HE)staining,immunohistochemistry(IHC)and real-time fluorescence quantitative PCR(qRT-PCR).The potential role of NOS was predicted using different bioinformatics methods.HE displayed that the mammary acini were collapsed and couple with infiltration inflammatory cell in the CM group.IHC indicated that the positive expression of neuronal NOS(nNOS),inducible NOS(iNOS),and endothelial NOS(eNOS)were presented in the cytoplasm of mammary epithelial cells.Compared to the Con group,the nNOS and eNOS mRNA expression levels were extremely significantly downregulated(P＜0.01),and iNOS mRNA expression levels was extremely significantly upregulated(P＜0.01)in mammary gland tissues of the CM groups.Bioinformatics analysis revealed that NOS might participate in CM occurrence in regulation of NO and amino acid synthesis and metabolism,ion transport.In conclusion,the above results provide a reference for revealing the function and regulatory mechanism of NOS in cow CM.关键词
临床型奶牛乳房炎/一氧化氮/一氧化氮合酶/生物信息学/功能分析Key words
Clinical mastitis/Nitric oxide/Nitric oxide synthase/Bioinformatics/Functional analysis分类
农业科学引用本文复制引用
李建富,张博皓,陈文丽,袁宝,张勇,赵兴绪,张全伟..一氧化氮合酶在奶牛临床型乳房炎乳腺组织中的表达、定位及功能分析[J].农业生物技术学报,2025,33(4):792-801,10.基金项目
甘肃省高等学校产业支撑计划(2024CYZC-30) （2024CYZC-30）
甘肃省创新驱动助力工程(GXH20240328-4) （GXH20240328-4）
国家自然科学基金(U21A20262) （U21A20262）
甘肃省研究生"创新之星"(2023CXZX-635) （2023CXZX-635）
