农业生物技术学报2025，Vol.33Issue(4)：814-822,9.DOI:10.3969/j.issn.1674-7968.2025.04.010
大通牦牛和阿什旦牦牛生长性状相关遗传标记的验证
Validation of Genetic Markers Related to Growth Traits in Datong Yak(Bos grunniens)and Ashidan Yak
摘要
Abstract
Connective tissue growth factor(CTGF),CREB regulated transcription coactivator 3(CRTC3),and transmembrane protein 18(TMEM18)play crucial roles in the growth and development of mammals.To identify genetic markers and gene polymorphisms associated with growth traits in the populations of Datong yak(Bos grunniens)and Ashidan yak,Sanger sequencing technology was used to detect and genotype 5 SNPs in the CTGF,CRTC3 and TMEM18 genes in Datong yak and Ashidan yak populations,and the association between the genotypes of polymorphic sites and growth traits were analyzed.The results showed that there were 2 genotypes at the G2511A loci of the CTGF gene in Datong yak and Ashidan yak populations,3 genotypes at the C86041T,G86075A loci of the CRTC3 gene,and 3 genotypes at the C1267T,C4447T loci of the TMEM18 gene.Among them,both the C86041T loci of the CRTC3 gene and the C4447T loci of the TMEM18 gene were moderately polymorphic(0.25＜PIC＜0.50).However,the Chi-square test revealed that none of the 5 SNPs reached Hardy-Weinberg equilibrium in the 2 yak populations,suggesting that the 2 populations have undergone long-term artificial selection and breeding.The association analysis of growth traits showed that the G2511A loci of the CTGF gene was significantly correlated with body height(P＜0.05);2 SNPs of the CRTC3 gene were significantly associated with body weight(P＜0.05),and the G86075A loci was significantly correlated with both body height and chest circumference(P＜0.05);2 SNPs of the TMEM18 gene were significantly associated with body weight(P＜0.05).Therefore,these growth trait-related SNP loci were correlated with the growth traits of Datong yaks and Ashidan yaks,and could be used as candidate genes for molecular marker-assisted selection in future breeding improvement.This study provides potential genetic markers for yak breeding improvement.关键词
大通牦牛/阿什旦牦牛/生长性状/遗传标记Key words
Datong yak/Ashidan yak/Growth trait/Genetic marker分类
畜牧业引用本文复制引用
马万浩,韩鹏,张有柱,张君,马晓玲,孙武,殷满财,景建武,胡广卫,李鸿康,赵寿保,马海福..大通牦牛和阿什旦牦牛生长性状相关遗传标记的验证[J].农业生物技术学报,2025,33(4):814-822,9.基金项目
财政部和农业部-国家现代农业产业技术体系(CARS-37) （CARS-37）
2022年度青海省"昆仑英才·高端创新创业人才"计划(领军人才)(QHKLYC-GDCXCY-2022-063) （领军人才）
青海省2021年牦牛育种联合攻关项目(青农财[2021)82号) （青农财[2021）
