农业生物技术学报2025，Vol.33Issue(4)：823-836,14.DOI:10.3969/j.issn.1674-7968.2025.04.011
迷迭香酸对奶山羊精液冷冻保存作用的研究
Effect of Rosemarinic Acid on the Cryopreservation of Dairy Goats(Capra hircus)Semen
摘要
Abstract
Sperm cells are highly susceptible to damage during semen cryopreservation,in which excessive free radicals cause oxidative stress that negatively affects the cellular system by increasing the production of reactive oxygen species.Therefore,the use of antioxidants in semen cryopreservation is crucial.Rosmarinic acid(RA)is a natural polyphenolic antioxidant with strong antioxidant activity.In this study,0,20,40,80 and 120 μmol/L RA was added to the semen cryopreservation diluent of dairy goats(Capra hircus)to test the sperm quality and sperm antioxidant capacity,and sperm plasma metabolic profiles of the spermatozoa of dairy goats were analyzed by LC-MS non-targeted metabolomics after freezing.The results showed that RA improved the quality of cryopreserved dairy goat spermatozoa and sperm antioxidant capacity,and 309 differential metabolites were detected between the control group and RA-treated group.Moreover,RA regulated the energy metabolism of spermatozoa through AMP-activated protein kinase(AMPK)signaling pathway,and activated or inhibited apoptosis-related proteins,such as B-cell lymphoma-2(Bcl-2),through PI3K-Akt signaling pathway.The present study demonstrated that the addition of 40 μmol/L RA could improve the effect of semen cryopreservation in dairy goats.This study provides a new idea and theoretical basis for the optimization of semen cryopreservation technology in dairy goats.关键词
迷迭香酸(RA)/精液冷冻保存/精液质量/抗氧化能力/代谢组学Key words
Rosemarinic acid(RA)/Semen cryopreservation/Semen quality/Antioxidant capacity/Metabolomics technology分类
农业科技引用本文复制引用
张佩,徐刚刚,袁媛,张建永,严天荣,彭忠宏,李宗儒,侯金星,安小鹏..迷迭香酸对奶山羊精液冷冻保存作用的研究[J].农业生物技术学报,2025,33(4):823-836,14.基金项目
陕西省重点研发计划(2024NC-ZDCYL-03-01 （）
2024NC-YBXM-082 （）
2024NC-GJHX-22 （）
2018ZDCXL-NY-01-02 （）
2020ZDLNY02-02 （）
2021ZDLNY02-01) （）
合水县科技计划项目(QY-HS-2024A-01) （QY-HS-2024A-01）
陕西省中央引导地方科技发展专项资金(2024ZY-CGZY-20和2024ZY-CGZY-22) （2024ZY-CGZY-20和2024ZY-CGZY-22）
杨凌示范区青年育种专家培育支持计划(202237) （202237）
杨凌职业技术学院自然科学研究项目(BG2022005) （BG2022005）
杨凌职业技术学院技术服务横向项目(0100136) （0100136）
