农业生物技术学报2025，Vol.33Issue(4)：857-866,10.DOI:10.3969/j.issn.1674-7968.2025.04.014
拮抗白术疫病病原菌的木霉菌的分离鉴定及其防效
Isolation and Identification of Trichoderma spp.Antagonizing the Pathogen of Atractylodes macrocephala Blight Disease and Their Control Effect
摘要
Abstract
In recent years,due to continuous cropping,the problem of pests and diseases in Atractylodes macrocephala has been severe,resulting in a decrease in yield and a decline in quality.Among them,the A.macrocephala epidemic caused by mold has caused serious losses to A.macrocephala production.As one of the most important biocontrol fungi,Trichoderma spp.has good antagonistic effects on various plant pathogens and can produce various bioactive substances with antagonistic effects.In this study,healthy rhizosphere soil and underground rhizomes of A.macrocephala continuous cropping land were taken as materials,to screen antagonistic Trichoderma strains using the pathogen AMPH-1 of Phytophthora sansomeana as an indicator bacterium.Through the inhibitory effect and antagonistic level of antagonistic,3 Trichoderma strains with better antibacterial effects,namely TPS-87,TPS-90,and TPS-99,were selected.The antibacterial rates against AMPH-1 were 77.19％,80.04％,and 78.49％.With morphological and molecular identification,strain TPS-87 was determined as T.afroharzianum,while TPS-90 and TPS-99 were both T.atroviride.When applying the suspension of TPS-87,TPS-90,and TPS-99 with a concentration of 1×106 cfu/mL,and with the inoculation amount of 150 mL,all 3 strains of Trichoderma had a good promoting effect on A.macrocephala seedlings,and the greenhouse control effects on A.macrocephala blight disease were 61.54％,53.85％,and 50％,respectively.This study provides biocontrol bacteria resources for the green prevention and control measures of A.macrocephala blight disease,and provides an important basis for the further development of biocontrol agents for the prevention and control of Chinese herbal medicine diseases.关键词
白术疫病/拮抗木霉菌/分离鉴定/促生作用/防治效果Key words
Atractylodes macrocephala blight disease/Antagonistic Trichoderma/Isolation and Identification/Promotive effect/Control effect分类
农业科技引用本文复制引用
王娇,刘晓宁,焦甜甜,沈凤英,刘大群,李亚宁..拮抗白术疫病病原菌的木霉菌的分离鉴定及其防效[J].农业生物技术学报,2025,33(4):857-866,10.基金项目
国家自然科学基金(32272605) （32272605）
