热带农业科学2025，Vol.45Issue(3)：30-36,7.DOI:10.12008/j.issn.1009-2196.2025.03.005
健康与连作发病香草兰根系分泌物差异成分筛选及其对尖孢镰刀菌的影响
Differential Root Metabolites Secreted by Newly Planted and Continuously Cropping Vanilla Orchids and Their Effects on Fusarium oxysporum
摘要
Abstract
Root exudates have a vital effect on the rhizosphere microbial community structure and composition.They are key driving factors for plant continuous cropping barriers.In the present research,gas chromatography-mass spectrometry(GC-TOF-MS)was used to evaluate the composition characteristics of root exudates secreted by newly planted healthy and continuously cropped vanilla orchids.Eighty-three metabolites were detected,including fatty acids,sugars,amino acids,phe-nolic acids,alcohols,amines,esters,glycosides,pyridines,pyrimidines,aldehydes,urea,etc.,were detected.Principal com-ponent analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)revealed that the root exudates of newly planted healthy and continuously cropped diseased vanilla orchids were clustered alongside and could be clearly dis-tinguished.Urea,lactose,diglyceride,citric acid,D-glycerol-1-phosphate,and sodium 4-hydroxybutyrate were the differen-tially abundant metabolites.The relative content of lactose in the roots of newly planted healthy vanilla orchids was signifi-cantly lower than that in the roots of continuously cropped diseased vanilla orchids.However,the relative contents of the other five differentially abundant metabolites were remarkably greater than those in the roots of continuously cropped diseased va-nilla orchids.The antagonistic tests revealed that increasing different concentrations of citric acid significantly inhibited Fusarium oxysporum sp.vanillae(Fov).However,an additional 200 mmol·L-1 lactose promoted Fov growth,followed by inhibition.关键词
香草兰/根系分泌物/代谢组学/尖孢镰刀菌Key words
vanilla orchid/root exudates/metabolites/Fusarium oxysporum分类
农业科技引用本文复制引用
邢诒彰,王洁,王辉,庄辉发,徐飞,赵青云..健康与连作发病香草兰根系分泌物差异成分筛选及其对尖孢镰刀菌的影响[J].热带农业科学,2025,45(3):30-36,7.基金项目
海南省自然科学基金(No.322QN404,No.321QN328) （No.322QN404,No.321QN328）
海南省重点研发计划项目(No.ZDYF2022XDNY268). （No.ZDYF2022XDNY268）
