热带农业科学2025，Vol.45Issue(3)：64-69,6.DOI:10.12008/j.issn.1009-2196.2025.03.010
低温贮藏对蝴蝶兰花粉活力的影响
Effects of Low-temperature Preservation on the Vitality of Phalaenopsis Pollen
摘要
Abstract
To solve the problem of the application of Phalaenopsis germplasm in time and space,the suitable low-temperat-ure storage conditions for Phalaenopsis pollen were screened,which provided technical support for hybrid breeding and germplasm conservation.The fresh pollen of Phalaenopsis'Xiaobanma'and'Zhengnong Huofenghuang'was used as test materials to observe the morphological characteristics of pollen under different storage temperatures,and the pollen viability was determined via TTC staining,pollination,and fruiting methods.The results indicated that after being treated at 25 ℃ and 4℃,the pollen of Phalaenopsis lost its viability after 15～30 days.After 360 days of storage and natural freezing at room temperature,the pollen treated at-20 ℃ and-80 ℃ had a darker surface color,harder texture,and larger volume than fresh pollen.TTC staining results indicated-20℃＜-80℃≤ fresh pollen.The pollination seed setting rate was basically consistent with the TTC test results.The pollen stored at-20℃ could not set seed regularly.The pollen of'Zhengnonghuofenghuang'could set seed regularly after 360 days of storage at-80℃ compared with the control,and the capsule developed well.The pollen of'Xiaobanma'could also set seed after 360 days of storage at-80℃,but compared with the control,the seed setting rate was lower,the capsule development was abnormal,and the seed quantity was lower.关键词
蝴蝶兰/杂交育种/种质资源/花粉活力/低温贮藏Key words
Phalaenopsis/hybrid breeding/germplasm resource/pollen viability/low-temperature preserved分类
农业科技引用本文复制引用
王世尧,张果,冯建,杨书才,杨录军,王俊..低温贮藏对蝴蝶兰花粉活力的影响[J].热带农业科学,2025,45(3):64-69,6.基金项目
河南省重点研发与推广专项(科技攻关)(No.232102111101) （科技攻关）
河南省林木种质资源建设项目(No.豫财环资[2023]60号). （No.豫财环资[2023]60号）
