四川环境2025，Vol.44Issue(2)：126-133,8.DOI:10.14034/j.cnki.schj.2025.02.016
HPLC-HRMS法测定小鼠组织中的邻苯二甲酸酯
Determination of Phthalate Esters in Mouse Tissues by HPLC-HRMS
摘要
Abstract
We established an ultrasound extraction-liquid chromatography-high resolution mass spectrometry/mass spectrometry to determine the content of phthalate esters(PAEs)in amniotic fluid,embryo,and placental samples of mouse.Through application experiments on various pre-treatment technologies for trace amounts of PAEs in biological tissues,the optimal conditions were determined as follows:Weighed 0.1 g samples and extracted them with 5 mL acetonitrile,and proceed with supersonic extraction;after centrifugation,purify the supernatant with a glass neutral alumina column,and quantitatively analyze the supernatant by liquid chromatography-high resolution mass spectrometry/mass spectrometry in full scan mode with the internal standard method.The results showed that the six PAEs exhibited good linearity within the mass concentration range of 2～50 µg/L,the correlation coefficient R was in the range of 0.9994 to 0.9999,and the detection limit was in the range of 2.7～4.2 μg/kg,the average recovery rate of the spiked samples was 88.2％～105％,and RSD was 9.0％～15％.With the method,sample pretreatment is simple,and test results are of high repeatability and accuracy.To sum up,the method is suitable for determination of PAEs in mouse tissues of low sample size and low content.关键词
超声提取/HPLC-HRMS/邻苯二甲酸酯/小鼠Key words
Ultrasound extraction/HPLC-HRMS/phthalate ester/mouse分类
资源环境引用本文复制引用
毛淑娟,竺美,唐文雅,林鑫,吴淼,杨秋燕,邓伟..HPLC-HRMS法测定小鼠组织中的邻苯二甲酸酯[J].四川环境,2025,44(2):126-133,8.基金项目
四川省生态环境科学研究院自有基金科技创新项目(2022-079). （2022-079）
