中国森林病虫2025，Vol.44Issue(2)：1-11,11.DOI:10.19688/j.cnki.issn1671-0886.20250014
黄栌枯萎病菌转录因子VdbZIP2的功能
Function of transcription factor VdbZIP2 in Verticillium dahliae,pathogen of verticillium wilt of smoke tree
摘要
Abstract
To deeply investigate the pathogenic mechanism of Verticillium dahliae,the causal agent of the devastating forest disease verticillium wilt of smoke tree,VdbZIP2,a bZIP transcription factor gene was deleted by PEG-mediated protoplast genetic transformation method.The biological traits of the mutants were analyzed,and the pathogenicity was determined.The results showed that knockout of VdbZIP2 seriously affected melanin synthesis,microsclerotia formation,cell wall stress sensitivity,and available nitrogen source utilization in V.dahliae.However,the mutants were fully pathogenic,indicating VdbZIP2 is not a virulence-essential gene.In summary,we conclude that the transcription factor VdbZIP2 of V.dahliae is involved in microsclerotia formation,adversity stress and nitrogen source utilization,which provides important clues for the subsequent in-depth analysis of the molecular regulatory network of VdbZIP2.关键词
大丽轮枝菌/黄栌枯萎病/bZIP转录因子/黑色素/微菌核/毒性Key words
Verticillium dahliae/verticillium wilt of smoke tree/bZIP transcription factor/melanin/microsclerotium/virulence分类
林学引用本文复制引用
李洒,王永林..黄栌枯萎病菌转录因子VdbZIP2的功能[J].中国森林病虫,2025,44(2):1-11,11.基金项目
国家自然科学基金项目"碳代谢阻遏途径调控黄栌枯萎病菌微菌核形成与营养胁迫应答的分子机制"(31971657) （31971657）
