首页|期刊导航|生物设计研究（英文）|Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strains

Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strains

Vincent J.Pennetti Peter R.LaFayette Wayne Allen Parrott

生物设计研究（英文）2025，Vol.7Issue(1)：5-15,11.

生物设计研究（英文）2025，Vol.7Issue(1)：5-15,11.DOI:10.1016/j.bidere.2025.100001

Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strains

Vincent J.Pennetti ¹Peter R.LaFayette ²Wayne Allen Parrott³

作者信息

1. Institute of Plant Breeding Genetics and Genomics,University of Georgia,Athens,GA,USA
2. Center for Applied Genetic Technologies,University of Georgia,Athens,GA,USA
3. Institute of Plant Breeding Genetics and Genomics,University of Georgia,Athens,GA,USA||Center for Applied Genetic Technologies,University of Georgia,Athens,GA,USA||Department of Crop and Soil Sciences,University of Georgia,Athens,GA,USA
折叠

摘要

关键词

Agrobacterium/CRISPR mediated base editing/Strain development/Disarming/Auxotrophy/Recombination deficiency/Plant transformation/Hairy roots

Key words

Agrobacterium/CRISPR mediated base editing/Strain development/Disarming/Auxotrophy/Recombination deficiency/Plant transformation/Hairy roots

引用本文复制引用

Vincent J.Pennetti,Peter R.LaFayette,Wayne Allen Parrott..Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strains[J].生物设计研究（英文）,2025,7(1):5-15,11.

基金项目

This material is based upon work supported by the Center for Bio-energy Innovation(CBI),U.S.Department of Energy,Office of Science,Biological and Environmental Research Program under Award Number ERKP886. We thank Dr.Thomas Jacobs(VIB-Ghent University,Ghent,Belgium)for providing the pEN-L4-PvirB-dCas9-CDA-UL-T3T-R1,pEN-L1-PJ23119-Bsa1-PglpT-sfGFP-TrrfB-Bsa1-Scaf-L2,and pEN-R2-SacB-L3 vectors （CBI）

Sebastian Cocioba for the promoter-rbs-spacer combination driving eforRed （）

Walter Dauksher for cloning the dicot RUBY expression vector （）

and Dr.Timothy Chappell for disarming Agrobacterium rhizogenes strain K599 through allelic replacement. （）

生物设计研究（英文）

访问量2

下载量0

段落导航