时珍国医国药2025，Vol.36Issue(5)：807-813,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-0502
基于JNK/P38/NF-κB信号通路探讨清解化攻方改善重症急性胰腺炎诱导的急性肺损伤的作用机制
Investigating the mechanism of Qingjie Huagong Decoction in attenuating acute lung injury in-duced by severe acute pancreatitis via the JNK/P38/NF-κB signaling pathway
摘要
Abstract
Objective To investigate the potential molecular mechanism by which Qingjie Huagong Decoction(QJHGD)alleviates in-flammation and regulates apoptosis in acute lung injury(ALI)induced by severe acute pancreatitis(SAP)through the JNK/P38/NF-κB signaling pathway.Methods Thirty-six SPF-grade male rats were randomly assigned to the sham-operated group,the model group,the Western medicine group,and the QJHGD low-,medium-,and high-dose groups(n=6 per group).Tissue and blood samples were collected from the experimental rats.The molecular mechanisms involving the JNK/P38/NF-κB signaling pathway were explored using hematoxylin-eosin(HE)staining,ELISA,IHC,RT-PCR,and Western blot(WB)analysis.Results Compared with the sham-operated group,the model group exhibited irregularly arranged and overlapping fibrotic structures in the lung tissue,enlarged pancreatic interlobular septa,acinar atrophy,and inflammatory cell infiltration.Pathological scores,as well as the concentrations of li-pase,amylase,IL-1β,TNF-α,and IL-6,were significantly elevated(P<0.05).The expression levels of JNK,P38,NF-κB,and AP1 mRNA and protein were also up-regulated(P<0.05).Compared with the model group,the Western medicine group and QJHGD groups with different doses showed reduced pathological damage in pancreatic and lung tissues,decreased concentrations of li-pase,amylase,IL-1β,TNF-α,and IL-6 in the lung tissues(P<0.05),and down-regulated expression levels of JNK,P38,NF-κB,and AP1 mRNA and protein(P<0.05).Conclusion QJHGD may exert protective effects on pancreatic and lung tissues by down-regulating inflammatory factors such as IL-1β,TNF-α,and IL-6,inhibiting the JNK/P38/NF-κB signaling pathway,and reducing apoptotic gene activation,thus mitigating apoptosis.QJHGD may serve as a potential complementary treatment for SAP-in-duced ALI.关键词
JNK/P38/NF-κB通路/清解化攻方/重症急性胰腺炎/急性肺损伤/炎症反应/细胞凋亡Key words
JNK/P38/NF-κB pathway/Qingjie Huagong Decoction/Severe acute pancreatitis/Acute lung injury/Inflamma-tory response/Apoptosis分类
医药卫生引用本文复制引用
李凯,冯敏超,朱晓东,班莹,刘锟荣,禤传凤,唐曦平,陈国忠..基于JNK/P38/NF-κB信号通路探讨清解化攻方改善重症急性胰腺炎诱导的急性肺损伤的作用机制[J].时珍国医国药,2025,36(5):807-813,7.基金项目
国家自然科学基金(82160890) （82160890）
国家中医优势专科建设项目(2024010) （2024010）
广西自然科学基金面上项目(2024GXNSFAA010246 （）
2020GXNSFAA297062) （）
广西中医药大学第一附属医院院级科研项目(2020QN024) （2020QN024）
中医药地方标准项目(2024001-02-05) （2024001-02-05）
广西中医药大学博士科研启动基金项目(2022BS029) （2022BS029）
