时珍国医国药2025，Vol.36Issue(5)：814-820,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-0503
中药复方强骨饮通过干预补体C3和SIRT5影响OPG/RANKL/RANK信号通路及骨微结构的实验研究
An experimental study on the effects of Qianggu Decoction on the OPG/RANKL/RANK signa-ling pathway and bone microstructure via modulating C3 and SIRT5
摘要
Abstract
Objective To investigate the effects of Qianggu Yin(QGY),/Bone-strengthening Decoction a traditional Chinese medicine(TCM)compound formula,on bone microstructure in osteoporosis(OP)and to explore its mechanism of regulating OPG/RANKL/RANK by intervening complements of C3 and SIRT5.Methods A postmenopausal OP mouse model was established via bilateral ovariec-tomy.Mice were randomly assigned to the sham group,the model group,or the QGY group.The QGY group received QGY via gavage,while the sham and model groups received an equal volume of normal saline.After eight weeks of treatment,the mice were sacrificed,and femoral tissues were collected for TRAP staining to assess osteoclast differentiation.Micro-CT and RT-qPCR were used to evaluate trabecular number,trabecular bone volume fraction,trabecular thickness,and trabecular spacing.Additionally,QGY-containing serum and control serum were prepared.The expression levels of C3,SIRT5,osteoprotegerin(OPG),and receptor activator of nuclear factor-κB ligand(RANKL)during osteoblast and osteoclast differentiation were assessed using qRT-PCR and Western blot(WB).Re-sults Compared with the sham group and model group,bone microstructure of mice in the QGY group was significantly improved.Addi-tionally,QGY inhibited complement C3,affected the succinylation key protein SIRT 5,and alleviated OP.In cell experiments,inhibited C3 gene expression was found to up-regulate the OPG expression,promote the expression of SIRT 5,a key protein for succinylation,and reduce the expression of RANKL.Conclusion QGY may exert its therapeutic effect on OP by modulating C3 levels and the succi-nylation-related protein SIRT5,thereby regulating OPG and RANKL expression.关键词
骨质疏松症/中药复方强骨饮/补体C3/SIRT5/OPG/RANKL/RANKKey words
Osteoporosis/Qianggu Decoction/C3/SIRT 5/OPG/RANKL/RANK分类
医药卫生引用本文复制引用
张徐,孟世龙,童铭豪,余阳,曹延广,张伟,史晓林,刘康..中药复方强骨饮通过干预补体C3和SIRT5影响OPG/RANKL/RANK信号通路及骨微结构的实验研究[J].时珍国医国药,2025,36(5):814-820,7.基金项目
国家自然科学基金(82274272) （82274272）
浙江省中医药重点研究项目(2022ZZ020) （2022ZZ020）
