时珍国医国药2025，Vol.36Issue(5)：851-860,10.DOI:10.70976/j.1008-0805.SZGYGY-2025-0508
凝结芽孢杆菌MZY531通过Wnt/β-catenin信号通路诱导人肝癌细胞铁死亡作用机制的研究
Investigating the mechanism of ferroptosis induced by bacillus coagulans MZY531 via the Wnt/β-catenin signaling pathway in human hepatocellular carcinoma cells
摘要
Abstract
Objective To investigate the mechanism by which Bacillus coagulans MZY531 induces ferroptosis in human hepatocellular carcinoma(HCC)cells through the Wnt/β-catenin signaling pathway.Methods The effect of B.coagulans MZY531 on the prolifera-tion of HepG2 and SK-Hep-1 HCC cells was assessed using the CCK-8 assay.The levels of malondialdehyde(MDA)and glutathi-one(GSH)in HCC cells were measured by the micro-colorimetric method.Intracellular Fe2+and lipid peroxide levels were detected u-sing a fluorescent probe assay.Western blot(WB)was performed to assess the expression of key ferroptosis-related proteins and Wnt/β-catenin pathway-associated proteins.Ferroptosis inhibitor Ferrostatin-1 and pathway inhibitor IWR-1 were used to further explore the intervention of the Wnt/β-catenin pathway in B.coagulans MZY531-induced ferroptosis.Results MZY531 inhibited the prolifera-tion of HCC cells,significantly increased intracellular Fe2+and lipid peroxide levels,elevated MDA expression,suppressed GSH expres-sion,and downregulated the expression of key ferroptosis-related proteins and Wnt/β-catenin pathway-associated proteins.Following the co-treatment with MZY531 and Ferrostatin-1,ferroptosis was markedly induced in HCC cells.Compared with the IWR-1 group,the MZY531+IWR-1 group exhibited further suppression of Wnt/β-catenin pathway-related proteins β-catenin,Cyclin D1,and c-Myc,along with downregulation of the ferroptosis-associated proteins GPX4 and SLC7A11.Conclusion B.coagulans MZY531 may induce ferroptosis in HCC cells by regulating the Wnt/β-catenin signaling pathway.关键词
凝结芽孢杆菌MZY531/肝癌/铁死亡/Wnt/β-catenin 信号通路Key words
Bacillus coagulans MZY531/Hepatocellular carcinoma/Ferroptosis/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
程颢颐,刘铭玥,陈佳欣,王丹丹,张学武,沈爱花..凝结芽孢杆菌MZY531通过Wnt/β-catenin信号通路诱导人肝癌细胞铁死亡作用机制的研究[J].时珍国医国药,2025,36(5):851-860,10.基金项目
国家自然科学基金(81760728) （81760728）
