时珍国医国药2025，Vol.36Issue(6)：1001-1006,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0601
丹玉和脉颗粒调控Nrf2/HO-1信号通路改善高血压大鼠肾损伤的研究
Investigating the effect of Danyu Hemai Granule(丹玉和脉颗粒)on renal injury in hyperten-sive rats via regulating the Nrf2/HO-1 signaling pathway
摘要
Abstract
Objective To observe the therapeutic effect of Danyu Hemai Granule(丹玉和脉颗粒,DYHMG)in spontaneously hyper-tensive rats(SHR)and explore its possible protective mechanism in Chinese herbal medicine(CHM)based on the Nrf2/HO-1 signa-ling pathway.Methods Eighteen 5-week-old male SHRs were adaptively fed for one week and then randomly assigned to three groups:the SHR group(gavage with distilled water),the DHG group(gavage with DHG at 42 g crude drug·kg-1 per day)and the valsartan group(gavage with valsartan at 30 mg·kg-1 per day),with 6 rats in each group.Six age and sex matched WKY rats gavaged with distilled water served as the control.All rats were gavaged once a day for 10 weeks,with blood pressure measured every 2 weeks.At the end of the 10th week,the renal tissues were collected.Pathological changes in the renal tissues were observed by HE and Masson staining.Western blot(WB)was used to detect the protein expressions of Nrf2,HO-1,and GPX4 in the renal tissues.Results Com-pared with the WKY group,the SHR group showed significantly elevated systolic blood pressure(P<0.001),enlarged glomerular vol-ume,vacuolization of renal tubular epithelial cells,and marked collagen fiber hyperplasia.Protein expressions of Nrf2,HO-1 and GPX4 in the renal tissues of rats in the SHR group were significantly decreased(P<0.001).Compared with the SHR group,DYHMG could lower the blood pressure(P<0.05),alleviated renal tubular vacuolization,ameliorated collagen hyperplasia,and significantly in-creased Nrf2 and HO-1 expression in the renal tissues(P<0.05).Conclusion DYHMG can reduce systolic blood pressure in SHRs,mitigate renal pathological damage and oxidative stress injury,and its mechanism may be associated with the regulation of the Nrf2/HO-1 pathway.关键词
丹玉和脉颗粒/高血压/肾脏损伤/核转录因子E2相关因子2/血红素加氧酶-1通路/氧化应激Key words
Danyu Hemai Granule(丹玉和脉颗粒)/Hypertension/Renal injury/Nuclear transcription factor E2-associated factor 2/Heme oxygenase-1 pathway/Oxidative stress分类
医药卫生引用本文复制引用
王亚妮,李建华,王明珠,芦波,符德玉,李云凤,马玉龙,陈晓喆,曹博莹,周训杰,桂明泰,姚磊..丹玉和脉颗粒调控Nrf2/HO-1信号通路改善高血压大鼠肾损伤的研究[J].时珍国医国药,2025,36(6):1001-1006,6.基金项目
国家自然科学基金(82274262 （）
82174130) （）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023065) （zyyzdxk-2023065）
上海市医院中药制剂产业转化协同创新中心项目 （）
上海中医药大学第十六批大学生创新活动计划项目(SHUTCM2023287) （SHUTCM2023287）
上海市白玉兰人才计划浦江项目(24PJD113) （24PJD113）
