时珍国医国药2025，Vol.36Issue(6)：1029-1034,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0605
基于VEGF/VEGFR-2和Ang-1/Ang-2/Tie-2信号通路探讨归肾丸合八珍汤对DOR大鼠卵巢的影响
Investigating the effect of Guishen Pill(归肾丸)plus Bazhen Decoction(八珍汤)on the ova-ries of DOR rats based on the VEGF/VEGFR-2 and Ang-1/Ang-2/Tie-2 signaling path-way
摘要
Abstract
Objective To investigate the possible mechanism of Guishen Pill(归肾丸)combined with Bazhen Decoction(八珍汤),ab-breviated as GSBZ,in treating diminished ovarian reserve(DOR).Methods Sixty SD rats were randomly assigned to the control group,cyclophosphamide(CTX)group,estradiol valerate(EV)group,and GSBZ low/medium/high-dose((GSBZ-L/GSBZ-M/GSBZ-H)groups.DOR model was established in all groups except the control group.After 21 days of treatment,vaginal exfoliative cytology with Liu's staining was used to observe the estrous cycle changes.Serum levels of E2,FSH and AMH were measured by ELISA.Ovari-an pathological changes and ultrastructure were observed through HE staining and transmission electron microscopy(TEM)respectively.Western blot(WB)was used to detect VEGF,VEGFR-2,Ang-1,Ang-2 and Tie-2 protein expressions.Results Compared with the control group,the CTX group showed disrupted estrous cycles,altered ovarian ultrastructure,increased serum FSH levels(P<0.05),decreased AMH and E2 levels(P<0.05),while both EV and GSBZ-L groups showed increased E2 levels(P<0.05).Ovarian VEGF in the CTX group,Ang-1 in the EV group,and VEGFR-2 protein levels in the GSBZ-M group were decreased(P<0.05),while VEGFR-2 protein levels in GSBZ-H group was increased(P<0.05).Compared with CTX group,EV and GSBZ groups exhibi-ted recovered follicular growth,reduced ovarian mitochondrial swelling with increased quantity,decreased serum FSH levels(P<0.05)in varied degrees,and increased serum AMH and E2 levels(P<0.05).Ovarian VEGF in the EV group,Ang-1 and Tie-2 in the GSBZ-M group,as well as VEGF,Ang-1 and Tie-2 in the GSBZ-H group were all increased(P<0.05).Conclusion GSBZ can improve serum hormone levels and ovarian structure in DOR rats while promoting angiogenesis,and its mechanism may be associated with the up-regulation of the VEGF/VEGFR-2 and Ang-1/Ang-2/Tie-2 signaling pathways.关键词
卵巢储备功能减退/归肾丸/八珍汤/血管生成/精血理论Key words
Diminished ovarian reserve(DOR)/Guishen pill(归肾丸)/Bazhen Decoction(八珍汤)/Angiogenesis/Theory of essence and blood分类
医药卫生引用本文复制引用
高同庆,尹燕,金志春,胡红霞,曾婕,王珺,蔡紫璨,吴锋英..基于VEGF/VEGFR-2和Ang-1/Ang-2/Tie-2信号通路探讨归肾丸合八珍汤对DOR大鼠卵巢的影响[J].时珍国医国药,2025,36(6):1029-1034,6.基金项目
国家自然科学基金青年项目(22301228) （22301228）
全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
