时珍国医国药2025，Vol.36Issue(6)：1040-1044,5.DOI:10.70976/j.1008-0805.SZGYGY-2025-0607
健脾疏肝消浊法对2型糖尿病大鼠糖脂代谢及GLUT-4、IRS-1表达的影响
Exploring the effects of Liver-soothing,Spleen-nourishing and Turbidity-resolving Thera-py(健脾疏肝消浊法)on glucolipid metabolism and GLUT-4 and IRS-1 expression in type 2 diabetic rats
摘要
Abstract
Objective To explore the pharmacological effects and underlying mechanisms of the Liver-soothing,Spleen-nourishing and Turbidity-resolving Therapy(健脾疏肝消浊法,LSTT)in treating type 2 diabetic rats based on the theory of visceral qi transfor-mation.Methods Twenty-one SD rats(SPF grade)were randomly divided into three groups:the normal group,model group,and Chi-nese herbal medicine(CHM)group,with 7 rats in each group.A type 2 diabetes mellitus(T2DM)rat model was established by intrap-eritoneal injection of streptozotocin(STZ)combined with a high-fat diet.The normal and model groups received normal saline via ga-vage,while the CHM group was treated with LSTT for 8 weeks.Body weight and food intake were recorded,and biochemical indicators were measured,including fasting blood glucose(FBG),2-hour postprandial blood glucose(2h PG),fasting serum insulin(FINS),total cholesterol(TC),and triglycerides(TG)levels.Pathophysiological changes of the pancreatic and hepatic tissues were observed u-sing HE staining.The expression levels of IRS-1 and GLUT-4 in tissues were detected by immunohistochemistry and Western blot(WB).Results LSTT reduced body weight,TG,and FBG levels in SD rats,promoted FINS secretion,and decreased the homeostasis model assessment of insulin resistance(HOMA-IR).Compared to the model group,the CHM group showed significant amelioration in pancreatic islet morphology,with more intact islet cell structures,and reduced cell vacuolation and necrosis.Hepatocyte cords in the CHM group were more orderly arranged,hepatic sinusoids were clearly visible,hepatocyte swelling and vacuolar degeneration were markedly reduced,and no inflammatory cell infiltration or fibrotic proliferation was observed.Additionally,protein expression levels of GLUT-4 and IRS-1 in the CHM group were significantly higher than those in the model group.Conclusion LSTT can effectively amel-iorate glucolipid metabolism disorders and improve insulin resistance in type 2 diabetic rats,potentially by regulating the expressions of IRS-1 and GLUT-4 in the hepatic tissues.关键词
2型糖尿病/健脾疏肝消浊/脏腑气化/糖脂代谢/葡萄糖转运蛋白受体-4/胰岛素受体底物-1Key words
Type 2 diabetes/Liver-soothing,Spleen-nourishing and Turbidity-resolving Method(健脾疏肝消浊法)/Viscer-al qi transformation/Glucolipid metabolism分类
医药卫生引用本文复制引用
姜玉华,陈曦,张冰晗,雷晶晶,荆业腾,杨金生..健脾疏肝消浊法对2型糖尿病大鼠糖脂代谢及GLUT-4、IRS-1表达的影响[J].时珍国医国药,2025,36(6):1040-1044,5.基金项目
中国中医科学院中医基础理论研究所基本科研业务费自主选题项目(YZX-202247) （YZX-202247）
中国中医科学院科技创新工程项目(CI2021A00104) （CI2021A00104）
