时珍国医国药2025，Vol.36Issue(6)：1050-1055,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-0609
柴胡-浙贝药对及其单体成分干预乳腺癌癌前病变的机制研究
Investigating the mechanism of Chaihu-Zhebei medicinal couplet(柴胡-浙贝药对)and its monomer components in intervening breast cancer precancerous lesions
摘要
Abstract
Objective To investigate the intervening effect and mechanism of Chaihu(Bupleuri Radix)-Zhebei(Bulbus Fritillariae Thunbergii)medicinal couplet(柴胡-浙贝药对,CZMC)and its monomeric components on rats with breast cancer precancerous le-sions.Methods Thirty female SD rats were randomly divided into the blank group,model group,tamoxifen group,Saikosaponin D-Peimine group and CZMC group,with 6 rats in each group.The rat model of breast cancer precancerous lesions was established by DM-BA combined with sequential estrogen-progesterone in the model group and drug intervention groups.Corresponding interventions were administered to each group.Body weight was recorded before and after modeling,and after administration.The contents of serum TNF-α,IL-1β and IL-6 were detected by ELISA.HE staining was used to observe the pathological changes of the mammary tissues.CD31-MVD was detected by IHC.The expressions of VEGF and HIF-1α were detected by RT-PCR,while the protein expressions of HIF-1α and Endostatin were assessed by Western blot(WB).Results Compared with the blank group,the model group exhibited severe ductal lesions in the mammary tissues,showing severe hyperplasia.Compared with the model group,all drug intervention groups showed increased body weight,improved ductal morphology in the mammary tissues with the manifestation of mild hyperplasia,and significant re-ductions in serum TNF-α,IL-1β,IL-6,CD31-MVD,VEGF and HIF-1α mRNA levels,and HIF-1α protein expression.En-dostatin protein expression was significantly elevated(P<0.05).Conclusion CZMC and its monomer components can alleviate the in-flammatory response in rats with breast cancer precancerous lesions and inhibit the formation of neovascularization in the mammary tis-sues.The mechanism may be associated with the modulation of VEGF/HIF-1α.关键词
"柴胡-浙贝"药对/柴胡皂苷D-贝母素甲/血管新生/乳腺癌/癌前病变Key words
Chaihu-Zhebei medicinal couplet(柴胡-浙贝药对)/Saikosaponin D-peimine/Angiogenesis/Breast cancer/Precancerous lesions分类
医药卫生引用本文复制引用
张文可,程旭锋,徐留燕,赵慧朵,刘琦..柴胡-浙贝药对及其单体成分干预乳腺癌癌前病变的机制研究[J].时珍国医国药,2025,36(6):1050-1055,6.基金项目
河南省科技攻关计划(222102310326) （222102310326）
河南省中医药科学研究专项(2022ZY1048) （2022ZY1048）
国家中医优势专科建设项目[国中医药医政函(2024)90号] （2024）
