时珍国医国药2025，Vol.36Issue(6)：1056-1064,9.DOI:10.70976/j.1008-0805.SZGYGY-2025-0610
黄连解毒汤调控SYK信号通路改善APP/PS1小鼠学习记忆能力的机制探讨
Exploring the mechanism of Huanglian Jiedu Decoction(黄连解毒汤)in ameliorating learning and memory ability of APP/PS1 mice through regulating the SYK signaling pathway
摘要
Abstract
Objective To investigate the ameliorating effect of Huanglian Jiedu Decoction(黄连解毒,HLJDD)on the cognitive func-tion of APP/PS1 mice with Alzheimer's disease(AD)model by modulating the SYK signaling pathway and its potential mechanisms.Methods Six-month APP/PS1 mice were randomly divided into the model group,HLJDD group,donepezil group and berberine group,with age-matched C57BL/6J mice serving as the control group.After60 days of administration,Morris Water Maze(MWM)and Novel Object Recognition(NOR)were used to evaluate the learning and memory ability of mice.Golgi staining and Congo red staining were employed to detect synaptic plasticity and Aβ plaque deposition respectively.RT-qPCR and Western blot(WB)were used to measure the expression of APP and its cleavage-related proteins(ADAM10,BACE1,PS1),inflammatory factors(TNF-α,IL-10)and SYK/PI3K/Akt/GSK3β signaling pathway.Results Behavioral results revealed that compared with the model group,the administration group exhibited shorter escape latency,increased target quadrant dwell time and more platform crossings,indicating enhanced ability of learning and memory.Golgi staining demonstrated improved synaptic plasticity,while Congo red staining showed reduced Aβ plaque dep-osition.RT-qPCR and WB indicated the expression of ADAM10 in administration group was increased,with promoted non-amyloido-genic APP cleavage,reduced Aβ levels,up-regulated anti-inflammatory factor IL-10,down-regulated pro-inflammatory factor TNF-α,and alleviated neuroinflammation.Meanwhile,HLJDD significantly increased the phosphorylation levels of SYK,PI3K,Akt and GSK3β.Conclusion HLJDD can improve the cognitive function of APP/PS1 mice by intervening the SYK/PI3K/Akt signaling path-way to reduce Aβ deposition,inhibit neuroinflammation,and strengthen synaptic plasticity.关键词
阿尔茨海默病/黄连解毒汤/脾酪氨酸激酶信号通路/APP/PS1小鼠/神经保护Key words
Alzheimer's disease/Huanglian Jiedu Decoction(黄连解毒汤)/SYK signaling pathway/APP/PS1 mice/Neu-roprotection分类
医药卫生引用本文复制引用
周家琪,黄子轩,游秋云,谭爱华..黄连解毒汤调控SYK信号通路改善APP/PS1小鼠学习记忆能力的机制探讨[J].时珍国医国药,2025,36(6):1056-1064,9.基金项目
国家自然科学基金青年基金(82405523) （82405523）
湖北省中医药管理局中医药科研项目(ZY2023Q025) （ZY2023Q025）
黄冈市卫生健康委黄冈市科技局中医药科研项目(黄卫发[2024]17号) （黄卫发[2024]17号）
