塔里木大学学报2025，Vol.37Issue(2)：31-38,8.DOI:10.3969/j.issn.1009-0568.2025.02.004
竹节秋海棠组织培养快繁技术体系的优化
Optimization of rapid propagation technology system of Begonia maculata Raddi in tissue culture
摘要
Abstract
In this experiment,tissue culture was conducted on the leaves of Begonia maculata Raddi,and the effect of different disinfection time on the sterilization effect of the explants was studied.Effects of different concentrations of hormones on adventitious bud induction in the first generation,adventitious bud proliferation in subsequent generations and adventitious root induction in Begonia maculata were experimented.The effects of different substrate ratio on transplanting in order to establish the system of rapid propagation technique of Begonia maculata Raddi in tissue culture.The results showed that the survival rate of leaves was 100%after disinfection with 75%ethanol for 30 s and 2%sodium hypochlorite solution for 5 min.MS+1.0 mg/L 6-BA+0.6 mg/L IBA was suitable for adventitious bud induction,and the induction rate was 100%.MS+0.1 mg/L 6-BA+1.0 mg/L IBA was suitable for adventitious bud proliferation and propagation,and the proliferation coefficient was 1.40.Then the 1/2MS+0.2 mg/L NAA was suitable for adventitious root growth,and 1/2MS+0.2 mg/L IBA was suitable for adventitious root elongation,both rooting rates were 100%.The survival rate of the substrate suitable for acclimation and transplanting was 100%on the conditions of turf∶vermiculite=1∶1.关键词
竹节秋海棠/叶片/组织培养/快繁体系Key words
Begonia maculata Raddi/leaf/tissue culture/rapid propagation system分类
园艺学与植物营养学引用本文复制引用
宋丹,杨德曌,韩凤,张红艳,闫芬芬..竹节秋海棠组织培养快繁技术体系的优化[J].塔里木大学学报,2025,37(2):31-38,8.基金项目
新疆生产建设兵团科技特派员创新创业计划项目(2021CB048) （2021CB048）
