生态环境学报2025，Vol.34Issue(4)：593-607,15.DOI:10.16258/j.cnki.1674-5906.2025.04.009
左氧氟沙星暴露对斑马鱼肠道微生物的影响
Effects of Levofloxacin Exposure on Intestinal Microbes of Zebrafish
摘要
Abstract
Levofloxacin is widely used in aquaculture,animal husbandry,veterinary medicine,and in human health as an antibiotic drug.This is a typical representation of a PPCPs.With the development and deepening of research on intestinal microbes,increasing evidence has shown that PPCPs can cause various diseases by disrupting gut microbiota homeostasis.Therefore,there is an urgent need to explore the relationship between levofloxacin and intestinal microbial homeostasis as well as the impact of levofloxacin on intestinal microbes.This study aimed to explore the effects of levofloxacin on the intestinal microbes of zebrafish and the resulting toxicity.Adult zebrafish were exposed to environmentally relevant concentrations of levofloxacin(0,10 μg·L-1,100 μg·L-1,1 mg·L-1,10 mg·L-1,and 100 mg·L-1)for 10 days,after which the morphological changes in zebrafish intestinal tissue were analyzed.The composition of the intestinal flora was analyzed using paraffin sectioning,HE staining,and 16S rDNA high-throughput sequencing.The results showed that zebrafish intestinal tissue sections revealed disordered intestinal gland structure,erosion of the mucosal layer,shedding of villi,and an increased number of inflammatory cells.These damaging effects increased with increasing concentrations of levofloxacin.16S rDNA high-throughput sequencing revealed that the abundances of Actinobacteria,Firmicutes,Bacteroidetes,and Proteobacteria in the intestinal bacteria of zebrafish decreased to 70%of that in the control group,and the microbial diversity index decreased significantly compared to that in the control group.In summary,levofloxacin can cause significant intestinal damage,immune and metabolic dysfunction,and other toxic effects in zebrafish,thereby reducing the abundance and diversity of the intestinal flora.This study provides a deeper understanding of the mechanisms underlying levofloxacin-induced gut toxicity,and offers a theoretical reference for the rational use of levofloxacin.关键词
左氧氟沙星/斑马鱼/肠道菌群/肠道稳态/环境毒理/抗生素Key words
levofloxacin/zebrafish/intestinal flora/intestinal homeostasis/environmental toxicology/antibiotic分类
环境科学引用本文复制引用
张亚平,莫璀玲,李迎鹏,徐棵..左氧氟沙星暴露对斑马鱼肠道微生物的影响[J].生态环境学报,2025,34(4):593-607,15.基金项目
国家自然科学基金项目(21978246 （）
22478119) （）
福建省自然科学基金项目(2014J01050) （2014J01050）
