生态环境学报2025，Vol.34Issue(4)：631-641,11.DOI:10.16258/j.cnki.1674-5906.2025.04.012
基于丛枝菌根的钒污染土壤修复
Remediation of Soil Polluted with Vanadium Via Arbuscular Mycorrhiza
摘要
Abstract
The pressing issue of vanadium(V)pollution in Chinese soils requires immediate attention,and combined plant and microbial remediation has been found to play a crucial role in the remediation of V-polluted soils.Microbial agents,such as arbuscular mycorrhizal fungi(AMF),which are ubiquitous in nature and form arbuscular mycorrhiza(AM)in most plants,have been used to remediate heavy metal pollution.However,the effectiveness and underlying mechanisms of AMF in mitigating V-polluted soil remain unclear.This study aimed to elucidate the patterns and underlying mechanisms of AMF remediation in V-polluted soils.In this study,dog tail grass(Setaria viridis)was used as a test plant and Rhizophagus irregularis served as the selected AMF strain.The experimental design included AMF-inoculated(+AMF)and non-inoculated(-AMF)groups,and pot experiments were conducted at three different levels of V pollution:mild(150 mg·kg-1),moderate(500 mg·kg-1),and severe(1000 mg·kg-1).The results showed that the soil V concentrations in the+AMF groups were significantly lower than those in the-AMF groups in all three cases,with mycorrhizal effects of 12,11,and 32%,respectively.This may be because AM significantly reduced the concentration of bioavailable vanadium,albeit with varying effects.Specifically,the order of the AM effects,from highest to lowest,was moderate pollution(59%),severe pollution(48%),and mild pollution(13%).AM significantly increased the biomass and V content of the plants and decreased the plant V concentration.In addition,AM improved the physical and chemical properties of the soil by increasing the weight mean diameter,mass geometric diameter,and total organic carbon concentrations,but also by decreasing the soil pH.The presence of AM increased the concentration of glomalin-related soil protein(GRSP),with the strongest AM effect observed for easily extractable GRSP,reaching 150%.Notably,AM led to an increase in the specific surface area of GRSP and a corresponding increase in the V concentration of GRSP,which reached a maximum of 1.2 mg·g-1 under heavy V loading in the+AMF groups.This value was 1.9 times higher than that of the corresponding soil V concentration,indicating the effective sequestration of V in the soil by GRSP within the matrix.These results highlight the significant potential of AM for the remediation of V-polluted soils,with GRSP emerging as a central component of this remediation process.关键词
丛枝菌根真菌/土壤钒污染/球囊霉素相关土壤蛋白/植物微生物联合修复Key words
arbuscular mycorrhizal fungi/soil polluted with vanadium/glomalin-related soil protein/plant-microorganism combined remediation分类
资源环境引用本文复制引用
张淑娟,陈昕龙,亓静凡,董月晓,于佳正,尤朝阳..基于丛枝菌根的钒污染土壤修复[J].生态环境学报,2025,34(4):631-641,11.基金项目
国家自然科学基金青年基金项目(42307474) （42307474）
国家自然科学基金项目(52370099) （52370099）
