中国组织工程研究2025，Vol.29Issue(32)：6821-6827,7.DOI:10.12307/2026.525
去铁胺减轻糖皮质激素抑制成骨分化的作用途径
Deferoxamine alleviates the inhibitory effect of glucocorticoids on osteogenic differentiation
摘要
Abstract
BACKGROUND:Deferoxamine exhibits multiple functions such as stem cell modulation,immune regulation,and promotion of angiogenesis and osteogenesis,but its role in the osteoinhibition induced by dexamethasone in osteoblasts remains unclear. OBJECTIVE:To investigate the effects of deferoxamine on osteoblasts treated with dexamethasone through the hypoxia-inducible factor 1α/vascular endothelial growth factor signaling pathway and to explore its potential mechanisms of action. METHODS:The proliferation of MC3T3-E1 cells treated with various concentrations of deferoxamine for 24,48,and 72 hours was assessed using the cell counting kit-8 assay to determine the optimal intervention concentration.There were control,dexamethasone,dexamethasone plus deferoxamine 10 μmol/L,and dexamethasone plus deferoxamine 20 μmol/L groups in the experiment.Cell counting kit-8 assay and flow cytometry were employed to evaluate the effect of deferoxamine on dexamethasone-induced cell proliferation and apoptosis.Alkaline phosphatase staining and activity assays were conducted to assess alkaline phosphatase levels in MC3T3-E1 cells.Alizarin red staining was used to observe the formation of mineralized nodules.Western blot was employed to detect the expression of osteogenic and signaling proteins. RESULTS AND CONCLUSION:(1)Deferoxamine showed no significant cytotoxicity to MC3T3-E1 cells within the range of 5-20 μmol/L and could ameliorate the inhibitory effects of dexamethasone on MC3T3-E1 cell proliferation and apoptosis.(2)Compared with the dexamethasone group,deferoxamine groups increased alkaline phosphatase activity and cell mineralization,and also significantly increased the protein expression of osteopontin,runt-related transcription factor 2,and alkaline phosphatase in MC3T3-E1 cells.(3)Deferoxamine also activated the hypoxia-inducible factor 1α/vascular endothelial growth factor pathway in dexamethasone-treated MC3T3-E1 cells.To conclude,deferoxamine can alleviate apoptosis in osteoblasts induced by dexamethasone treatment,maintain the vitality of osteoblasts by activating the hypoxia-inducible factor 1α/vascular endothelial growth factor signaling pathway,and promote their proliferation,which may help delay the progression of steroid-induced osteonecrosis of the femoral head.关键词
MC3T3-E1细胞/股骨头坏死/成骨分化/糖皮质激素/低氧诱导因子1α/血管内皮生长因子/去铁胺Key words
MC3T3-E1 cells/osteonecrosis of the femoral head/osteogenic differentiation/glucocorticoids/hypoxia-inducible factor 1α/vascular endothelial growth factor/deferoxamine分类
医药卫生引用本文复制引用
唐昊旭,梁英杰,李策,丁鹏霖,钱闵龙,袁伶俐..去铁胺减轻糖皮质激素抑制成骨分化的作用途径[J].中国组织工程研究,2025,29(32):6821-6827,7.基金项目
安徽省高等学校自然科学研究项目(KJ2021A0756),项目负责人:袁伶俐 （KJ2021A0756）
蚌埠医学院2023年度研究生科研创新计划(Byycxz23046),项目负责人:唐昊旭 Natural Science Research Project of Higher Education Institutions in Anhui Province,No.KJ2021A0756(to YLL) （Byycxz23046）
Bengbu Medical University 2023 Graduate Student Research and Innovation Program,No.Byycxz23046(to THX) （to THX）
