中国组织工程研究2025，Vol.29Issue(32)：6836-6842,7.DOI:10.12307/2025.941
亚精胺/精胺N1-乙酰转移酶1参与血管平滑肌细胞钙化
Spermidine/spermine N1-acetyltransferase 1 participates in vascular smooth muscle cell calcification
摘要
Abstract
BACKGROUND:Polyamines play a crucial role in tissue calcification.Spermidine/spermine N1-acetyltransferase 1(SAT1),as a key rate-limiting enzyme regulating intracellular polyamine metabolism,has been associated with various pathological processes.However,its role in vascular calcification remains unclear. OBJECTIVE:To investigate the role of SAT1 in rat vascular smooth muscle cell calcification. METHODS:(1)Bioinformatics analysis:Differential expression of SAT1 in human carotid atherosclerotic plaques and their surrounding healthy carotid artery tissues were using GEO datasets.PanglaoDB database was used to analyze SAT1 expression abundance and localization across different cell types through single-cell sequencing.(2)Rat vascular smooth muscle cells were divided into three groups:a control group cultured in DMEM medium,a calcification group induced by DMEM medium containing 10 mmol/L β-glycerophosphate sodium and 3 mmol/L calcium chloride,and the 50,100 μmol/L diacetylaminotriazamidine groups treated with the SAT1 inhibitor,diacetylaminotriazamidine,in addition to the calcification medium.After 7-10 days of culture,alizarin red S staining was performed,and cellular calcium content and alkaline phosphatase activity were assessed.Western blot was used to detect the protein expression of Runt-related transcription factor 2,bone morphogenetic protein 2,alpha-smooth muscle actin,and SAT1.Immunofluorescence staining was conducted to examine the expression of Runt-related transcription factor 2 and SAT1. RESULTS AND CONCLUSION:(1)Bioinformatics analysis revealed significantly upregulated expression of SAT1 and Runt-related transcription factor 2(P<0.05)in carotid atherosclerotic plaques compared with healthy carotid tissues(P<0.05).Single-cell sequencing database analysis confirmed SAT1 expression in vascular smooth muscle cells.(2)Compared with the control group,the calcification group showed significantly increased Runt-related transcription factor 2,bone morphogenetic protein 2,SAT1,calcium content,and alkaline phosphatase activity,while alpha-smooth muscle actin expression was significantly decreased(all P<0.05).Compared with the calcification group,the 50 and 100 μmol/L diacetylaminotriazamidine groups showed significantly decreased Runt-related transcription factor 2,bone morphogenetic protein 2,calcium content,and alkaline phosphatase activity,while alpha-smooth muscle actin expression was significantly increased(all P<0.05).(3)Immunofluorescence experiments demonstrated that compared with the calcification group,the expression intensity of Runt-related transcription factor 2 was significantly reduced in the 50 and 100 μmol/L diacetylaminotriazamidine groups.Overall,SAT1 may promote vascular smooth muscle cell calcification by upregulating Runt-related transcription factor 2 expression.关键词
大鼠血管平滑肌细胞/亚精胺/精胺N1-乙酰转移酶1(SAT1)/Runt相关转录因子2/血管钙化/生物信息学/平滑肌肌动蛋白Key words
rat vascular smooth muscle cells/spermidine/spermine N1-acetyltransferase 1(SAT1)/Runt-related transcription factor 2/vascular calcification/bioinformatics/smooth muscle actin分类
医药卫生引用本文复制引用
王凯茹,贾绍斌,付士哲,李家辉,燕茹,马玉茹,师波,冶从燕,闫瑞,丛广志..亚精胺/精胺N1-乙酰转移酶1参与血管平滑肌细胞钙化[J].中国组织工程研究,2025,29(32):6836-6842,7.基金项目
国家自然科学基金项目(82260086),项目负责人:贾绍斌 （82260086）
宁夏心脏瓣膜疾病规范化诊疗技术应用示范(2022FRD05046),项目负责人:贾绍斌 National Natural Science Foundation of China,No.82260086(to JSB) （2022FRD05046）
Ningxia Heart Valve Disease Standardized Diagnosis and Treatment Technology Application Demonstration,No.2022FRD05046(to JSB) （to JSB）
