中国组织工程研究2025，Vol.29Issue(32)：6851-6857,7.DOI:10.12307/2025.943
通督活血汤抑制巨噬细胞炎症延缓大鼠椎间盘退变的机制
Mechanism by which Tongdu Huoxue Decoction inhibits macrophage inflammation to delay intervertebral disc degeneration in rats
摘要
Abstract
BACKGROUND:Intervertebral disc degeneration is the main pathological factor causing low back pain,which is closely related to macrophage-mediated immune inflammation.Tongdu Huoxue Decoction is a proven prescription for the treatment of intervertebral disc degeneration,but it is still unclear whether it can treat intervertebral disc degeneration by regulating the polarization phenotype of macrophages to inhibit inflammation in intervertebral disc tissue. OBJECTIVE:To investigate the regulatory effects of Tongdu Huoxue Decoction on the expression of macrophage-related inflammatory factors in intervertebral disc tissues of rats,as well as its mechanisms for the treatment of intervertebral disc degeneration. METHODS:Twenty-four Sprague-Dawley rats were randomly divided into sham operation group,model group,and Tongdu Huoxue Decoction group,with eight rats in each group.An intervertebral disc degeneration model was established using the annulus fibrosus puncture method in the latter two groups.On the 1st postoperative day,10.8 g/kg Tongdu Huoxue Decoction was given by gavage in the Tongdu Huoxue Decoction group,and the same dose of saline was given by gavage in the sham operation group and the model group,once a day.After 4 weeks of intervention,histopathological changes in the intervertebral disc tissues were assessed using hematoxylin-eosin.Immunohistochemistry and quantitative real-time PCR were performed to detect the relative expression levels of CD68,CD206,interleukin 1β,interleukin 10,type II collagen,and matrix metalloproteinase 13 proteins or mRNA in the intervertebral disc tissues. RESULTS AND CONCLUSION:(1)Hematoxylin-eosin staining results revealed that the model group exhibited a significant decrease in intervertebral disc height,disorganized annulus fibrosus structure with fissures,and unclear demarcation between the nucleus pulposus and the annulus fibrosus.The Tongdu Huoxue Decoction group showed organized arrangement of the annulus fibrosus with pyknosis.(2)Immunohistochemical results demonstrated that,compared with the model group,the Tongdu Huoxue Decoction group had significantly lower expressions of CD68,interleukin 1β,and matrix metalloproteinase 13,and significantly higher expressions of CD206,type II collagen and interleukin 10(P<0.05 or P<0.01).(3)qPCR results showed that there were significant differences in the mRNA expression of CD68,CD206,interleukin 1β,matrix metalloproteinase 13,type II collagen,and interleukin 10 between the three groups(P<0.001).To conclude,Tongdu Huoxue Decoction can improve intervertebral disc degeneration in the rat model of intervertebral disc degeneration,and its mechanism is associated with the inhibition of macrophage-related inflammatory responses in the intervertebral discs.关键词
通督活血汤/椎间盘退变/髓核/巨噬细胞/炎症/机制/工程化组织构建Key words
Tongdu Huoxue Decoction/intervertebral disc degeneration/nucleus pulposus/macrophages/inflammation/mechanism/engineered tissue construction分类
医药卫生引用本文复制引用
鄢来军,葛海雅,汪正明,杨宗睿,牛立峰,詹红生..通督活血汤抑制巨噬细胞炎症延缓大鼠椎间盘退变的机制[J].中国组织工程研究,2025,29(32):6851-6857,7.基金项目
南通市卫生和计划生育委员会科研课题(QA2021025),项目负责人:鄢来军 （QA2021025）
上海市临床重点专科建设项目(shslczdzk03901),项目负责人:詹红生 Nantong Municipal Health and Family Planning Commission Scientific Research Project,No.QA2021025(to YLJ) （shslczdzk03901）
Shanghai Clinical Key Specialty Construction Project,No.shslczdzk03901(to ZHS) （to ZHS）
