亚太传统医药2025，Vol.21Issue(4)：12-16,5.DOI:10.11954/ytctyy.202504003
电针肺俞穴及足三里对COPD模型大鼠抗炎效果及机制研究
Study on the Anti-Inflammatory Effects and Mechanisms of Electroacupuncture at Feishu(BL13)and Zusanli(ST36)Acupoints in a COPD Rat Model
摘要
Abstract
Objective:To investigate the therapeutic effects and mechanisms of electroacupuncture on a rat model of chronic obstructive pulmonary disease(COPD).Methods:Forty Sprague-Dawley(SD)rats were randomly divided into four groups:control,model,medication,and electroacupuncture,with 10 rats in each group.The model group was in-duced with COPD by repeated intratracheal instillation of lipopolysaccharide(LPS),while the control group received saline instead.The control group received no treatment,the medication group was treated with dexamethasone(5 mg/kg),and the electroacupuncture group received treatment at the bilateral BL13(Feishu)and ST36(Zusanli)acupoints with an electroacupuncture frequency of 4/20 Hz for four weeks.After treatment,the rats were anesthetized,and bron-choalveolar lavage fluid(BALF)was collected for cell classification and counting.Lung tissues were collected for hema-toxylin-eosin(HE),and Masson's trichrome staining.The levels of IL-6,TNF-α,IL-1β,HO-1,SOD,and MDA in the BALF were measured.Western blot analysis was used to detect the expression levels of p-NF-κB,NF-κB,and I-κBαproteins in lung tissues.Results:Compared with the control group,the model group showed a significant increase in in-flammatory and oxidative stress-related factors(P＜0.01),with HE and Masson's staining revealing collagen deposi-tion and alveolar structure disruption.Compared with the model group,the medication and electroacupuncture groups showed significant inhibition of NF-κB pathway phosphorylation(P＜0.01),promotion of I-KBα expression(P＜0.05),a significant reduction in the levels of IL-6,TNF-α,IL-1β,and MDA(P＜0.001),and a significant increase in the levels of HO-1 and SOD(P＜0.001).Additionally,electroacupuncture and medication reversed LPS-induced alveolar structural damage and collagen deposition.Conclusion:Electroacupuncture exerts therapeutic effects on COPD by inhibiting NF-κB protein phosphorylation,thereby showing potential as a treatment for COPD.关键词
慢性阻塞性肺疾病/电针/NF-κB/分子机制Key words
Chronic Obstructive Pulmonary Disease/Electroacupuncture/NF-κB/Molecular Mechanism分类
医药卫生引用本文复制引用
王歌,宋永达,潘富伟,谢雨辰..电针肺俞穴及足三里对COPD模型大鼠抗炎效果及机制研究[J].亚太传统医药,2025,21(4):12-16,5.基金项目
国家自然科学基金项目(80874513) （80874513）
河南省中医药科学研究专项课题(2022ZY2023) （2022ZY2023）
中华医学会医学教育分会和全国医学教育发展中心医学教育研究课题项目(2023B099) （2023B099）
河南省中医药科学研究专项课题(2024ZY3059) （2024ZY3059）
河南省中医药科学研究专项课题(2024ZY3050) （2024ZY3050）
