亚太传统医药2025，Vol.21Issue(4)：17-22,6.DOI:10.11954/ytctyy.202504004
冬虫夏草菌定殖对小金蝠蛾幼虫体内能量物质跨膜转运效率的影响研究
Research on the Impact of Ophiocordyceps Sinensis Fungus Colonization on the Transmembrane Transport Efficiency of Energy Substances in the Larvae of Hepialus Xiaojinensis
摘要
Abstract
Objective:To reveal the maintenance mechanism of the coexistence of Ophiocordyceps sinensis fungus within the larvae of hepialus xiaojinensis moths,so as to provide references for improving breeding efficiency and promoting the sustainable renewal of resources.Methods:Present study was conducted from the perspective that host insect was the sole source of nutrients for Ophiocordyceps sinensis.Non-targeted mass spectrometry(NTSM)was used to identi-fy the main nutrient types required for the formation of Ophiocordyceps sinensis after infecting the larvae,and the main nutrient substrates distribution and transport efficiency in host's were evaluated.The host translocation efficiency for the main nutrient substrates required by the fungus in the larvae was assessed,and then the maintenance mechanism of the symbiotic phenomenon between the fungus and the insect under the background of the host's shared nutritional re-sources was revealed.Results:After being infected by Ophiocordyceps sinensis,the number of annotations of the host ABC transport pathway increased significantly.The types and contents of transferred nutrient substrates were also sig-nificantly increased.L-Arabinose and L-arginine were up-regulated more than 2-fold(P＜0.05).D-galactofuranose and Hydroxyproline were up-regulated more than 1.5-fold.The differences in transport substrates were then compared be-tween hemolymph and fat body after fungal infection of the hosts and showed a significant increase in hemolymph(P＜0.05).In addition,in isolated fat body cultures treated with L-arginine,the L-arginine content in the medium was re-duced by(0.06±0.04)mg/mL compared to uninfected,and the concentration of L-arginine in the buffer increased sig-nificantly to(0.11±0.014)mg/mL after incubation of fat bodies with PBS.This suggested a significant increase in the efficiency of bidirectional transmembrane transport of liposomes(P＜0.05).Conclusion:It is clear that the increased efficiency of ABC transmembrane transport helps maintain the energy requirements of Ophiocordyceps sinensis.It transfers energy stored in the host body from fat to hemolymph via transmembrane transport,and is also an important factor in insect-fungus coexistence.关键词
冬虫夏草/小金蝠蛾幼虫/虫菌共存/跨膜转运Key words
Chinese Cordyceps/Larvae of Hepialus Xiaojinensis/Insect-Fungus Coexistence/Transmembrane Transport分类
医药卫生引用本文复制引用
卫秋阳,岳勇,贺元川,陈仕江..冬虫夏草菌定殖对小金蝠蛾幼虫体内能量物质跨膜转运效率的影响研究[J].亚太传统医药,2025,21(4):17-22,6.基金项目
重庆市科技局基础研究与前沿探索专项(cstc2021jcyj-msxmX0762) （cstc2021jcyj-msxmX0762）
重庆市科技局科研院所绩效激励引导专项(基本科研业务费)项目(cstc2023jxjl-jbkyX0002) （基本科研业务费）
重庆市科技局技术创新与应用发展专项(cstb2022titad-pgx0067) （cstb2022titad-pgx0067）
重庆英才计划创新创业领军人才项目(2022) （2022）
重庆市博士后研究项目(2021XM3003) （2021XM3003）
重庆市中药研究院基本科研项目(jbky20210024) （jbky20210024）
