医学信息2025，Vol.38Issue(7)：141-144,4.DOI:10.3969/j.issn.1006-1959.2025.07.028
参葛补肾胶囊中淫羊藿的薄层色谱鉴别研究
Study on Thin Layer Chromatography Identification of Epimedium brevicornu Maxim.in Shenge Bushen Capsule
陈沫 1郝娟 1王涵斌 1王翻红 1李珍1
作者信息
- 1. 新疆华春生物药业股份有限公司研发中心,新疆 乌鲁木齐 830000
- 折叠
摘要
Abstract
Objective To establish an operable,specific,accurate and reliable quality control method for Epimedium brevicornu Maxim.in Shenge Bushen capsule by determining the optimal thin layer chromatography conditions.Methods With Epimedium brevicornu Maxim.reference medicinal materials and icariin reference substance as index components,the extract of Shenge Bushen capsule was spotted on the chromatographic plate,and the effects of developing agent and test solvent on thin layer chromatography analysis were investigated to screen out the best thin layer chromatography conditions.Results The silica gel G thin layer plate was used,with chloroform-methanol-water(7∶2.5∶0.25)as the developing agent,sprayed with 1%aluminum chloride ethanol solution,and examined under a 365 nm ultraviolet lamp.The chromatographic spots were clear,the separation effect of each component was good,and the fluorescent spots of the same color were shown at the corresponding positions of the control medicinal materials.Conclusion This method can be used for the qualitative identification of Epimedium brevicornu Maxim.in Shenge Bushen capsule.关键词
参葛补肾胶囊/淫羊藿/薄层色谱鉴别/展开剂/显色剂Key words
Shenge Bushen capsule/Epimedium brevicornu Maxim./Thin layer chromatography identification/Expander/Chromogenic agent分类
医药卫生引用本文复制引用
陈沫,郝娟,王涵斌,王翻红,李珍..参葛补肾胶囊中淫羊藿的薄层色谱鉴别研究[J].医学信息,2025,38(7):141-144,4.
