应用化学2025，Vol.42Issue(3)：321-329,9.DOI:10.19894/j.issn.1000-0518.240222
基于ZnS修饰电极构建光电化学生物传感器测定L-半胱氨酸
Construction of a Photoelectrochemical Biosensor Based on ZnS-Modified Electrode for the Determination of L-Cysteine
摘要
Abstract
L-Cysteine(L-Cys)levels in serum are correlated with many diseases,and their highly sensitive detection is of great significance.A novel strategy for selective detection of L-Cys based on photoelectrochemical catalytic oxidation was developed utilizing the electrochemical deposition of ZnS nanomodified electrodes.Under light irradiation,ZnS nanoparticle underwent a process of electron-hole pair formation.The thiol groups(—SH)on L-Cys in the solution provided electrons to occupy the holes,and the photoinduced electrons on the conduction band were injected into the ITO electrode to form an anodic photocurrent.Meanwhile,the L-Cys in the solution underwent a reduction process,whereby electrons were transferred to form cystine containing disulfide bridge(—S—S—),which then underwent a photoelectrochemical catalytic oxidation process.The photoelectric conversion efficiency was improved.The photocurrent of the sensor based on the photochemical catalytic oxidation mechanism increased linearly in the concentration range of 2～50 μmol/L,and obtained the detection limit of 0.67 μmol/L.This method was applied to determine the content of L-Cys in human serum,and the recovery rates were in the range of 97.6％to 104.6％.关键词
光电化学分析/硫化锌/L-半胱氨酸Key words
Photoelectrochemical analysis/ZnS/L-Cysteine分类
化学引用本文复制引用
陈结霞,刘卓尔,鹿长宁,杨可欣,陈紫艳,尉艳..基于ZnS修饰电极构建光电化学生物传感器测定L-半胱氨酸[J].应用化学,2025,42(3):321-329,9.基金项目
安徽高校自然科学研究重大项目(No.2023AH040250)、安徽高校自然科学研究重点项目(No.KJ2021A0842)和大学生科研资助金项目(No.WK2023XS43)资助 Supported by the Research Fund for Major Natural Science Research Projects in Universities of Anhui Province(No.2023AH040250),the University Natural Science Research Project of Anhui Province(No.KJ2021A0842)and the Undergraduate Research Grants Programme(No.WK2023XS43) （No.2023AH040250）
