应用化学2025，Vol.42Issue(3)：345-352,8.DOI:10.19894/j.issn.1000-0518.240242
靶向敲除CDK2致人黑素瘤移植瘤的蛋白质组改变
Proteomic Alterations in Xenograft of Human Melanoma by Targeting Knockout of Cyclin-Dependent Kinase 2
摘要
Abstract
Human melanoma is a cutaneous melanoma with high morbidity and mortality,especially for advanced malignant melanoma which pose a seriously threat to human health.Dysregulation of cell cycle of human melanoma cell is sufficient to cause human melanoma.Among the cyclin-dependent kinases(CDKs),CDK2 plays an important role in regulation of cell cycle.However,how CDK2 regulate the proteome of human melanoma cell remains unknown.In this study,the proteomic alterations in human melanoma xenograft by targeting knockout of CDK2 were studied.The results showed that a total of 185 differentially expressed proteins(DEPs)were identified as significantly changed DEPs,68 DEPs were down-regulated and 117 DEPs were up-regulated in CDK2 knockout(CDK2-KO)group compared with tumor group.A total of 203 DEPs were identified as significantly changed DEPs,98 DEPs were down-regulated and 105 DEPs were up-regulated in CDK2-KO group compared with CDK2 knockout negative(CDK2-NC)group after human melanoma xenograft was infected with recombinant sgCDK2-108 lentivirus.Rps29,Adrm1,Fam162a and Rps15 were same down-regulated DEPs,Obp1b,Serpinb1a,Ttr and Slc9a3r1 were same up-regulated DEPs.These DEPs mainly referred to biological processes such as regulation of peptidase activity,regulation of endopeptidase activity,negative regulation of hydrolase activity.This study reveals the role of CDK2 in regulation network and biological function in human melanoma and provides a novel evidence for targeting CDK2 in human melanoma therapy.关键词
人黑色素瘤/CDK2/蛋白质组/移植瘤Key words
Human melanoma/CDK2/Proteome/Xenograft分类
化学引用本文复制引用
刘厚广,李铮,霍珊珊,魏琼玲,张海梅,吴剑宾,蒋雅雯..靶向敲除CDK2致人黑素瘤移植瘤的蛋白质组改变[J].应用化学,2025,42(3):345-352,8.基金项目
福建省卫生健康科技计划项目-省医学创新课题(No.2021CXB027)资助 Supported by Fujian Provincial Health Technology Project(No.2021CXB027) （No.2021CXB027）
