中国动物检疫2025，Vol.42Issue(3)：21-26,6.DOI:10.3969/j.issn.1005-944X.2025.03.004
四川省甘孜州牦牛4种腹泻病原调查及病毒宏基因组学检测
Investigation on Four Diarrhea Pathogens in Yaks in Ganzi Prefecture of Sichuan Province and Metagenomics Testing of Viruses
摘要
Abstract
In order to investigate major pathogens causing diarrhea in calf yaks and composition of the viruses in Ganzi Prefecture,Sichuan Province,216 fecal samples were collected from calf yaks with diarrhea to detect bovine viral diarrhoeavirus(BVDV),bovine coronavirus(BCoV),Giardia and Cryptosporidium by real-time fluorescence quantitative PCR,and the viruses were sequenced using metagenomics technology.The results showed that the individual pathogen positive rate was 52.78％for all the samples,23.61％,6.02％,17.59％and 15.74％for BVDV,BCoV,Giardia and Cryptosporidium,respectively,the species of pathogens detected were subject to different districts/counties where the samples were collected;the mixed infection rate was 15.79％,including double and triple infection of 10.53％and 5.26％,respectively;and 15 kinds of viruses were detected,including BVDV,BCoV,bovine herpesvirus serotype 4(BHV-4),bovine kobuvirus(BKV),bovine rotavirus(BRV)and bovine astrovirus(BoAstV)that contributed to the diarrhea of yaks.In conclusion,the yak diarrhea in Ganzi Prefecture was mainly caused by BVDV,BCoV,Giardia and Cryptosporidium,where mixed infection was available.In addition,BHV-4,BRV,BoAstV and BKV were also prevalent in the yaks.Epidemiological investigation and appropriate prevention and control measures were recommended for yak diarrhea.关键词
牛病毒性腹泻病毒/牛冠状病毒/贾第虫/隐孢子虫/病毒宏基因组学Key words
BVDV/BCoV/Giardia/Cryptosporidium/viral metagenomics分类
畜牧业引用本文复制引用
李劲,潘瑶,肖晨冬,吴建平,杨怀珍,计慧姝,罗勇,扎西泽仁,奉奇,蓝岚..四川省甘孜州牦牛4种腹泻病原调查及病毒宏基因组学检测[J].中国动物检疫,2025,42(3):21-26,6.基金项目
四川省苗子工程重点项目(2023JDRC0121) （2023JDRC0121）
四川省转移支付科技计划项目(220022) （220022）
