免疫层析技术在布鲁氏菌病检测中的研究现状OA

Brucellosis is a zoonotic disease caused by Brucella,posing a serious threat to human health and livestock industry,which should be prevented and controlled through rapid and accurate diagnosis as a priority.Immunochromatographic assay(ICA),as a rapid detection technique combining specific antigen-antibody reaction with chromatographic principles,is accessible and rapid with visualized results,and suitable for on-site detection of brucellosis.It is usually developed based on 3 kinds of Brucella antigens including lipopolysaccharide(LPS),whole bacterial protein and outer membrane protein.The LPS is the antigen most commonly used for the development of technology for detection of Brucella antibodies,the whole bacterial protein can improve the sensitivity of detection,and the outer membrane protein has good specificity and can be effectively differentiated from that of other bacteria.The ICA is currently used for initial screening of brucellosis,and could be combined with ELISA and fluorescence polarization assay(FPA)to confirm any cases,as its results are easily affected and limited by preparation process,storage conditions,usages and other conditions.In the study,its application in brucellosis detection was reviewed from the aspects of antibody and pathogen detection,its characteristics and selection of antigen and antibody were analyzed,the trend of its integration and development with new technologies and instruments was expected,with a view to providing a technical support for the prevention and control of brucellosis in China,and a reference for researches on rapid detection technology for other animal diseases.