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肉鸡屠宰及零售环节沙门氏菌污染的定性和定量检测方法比较

张思宇高玉斌曲志娜王君玮赵格黄保续刘俊辉王娟王琳刘娜张喜悦宋时萍赵建梅黄秀梅

中国动物检疫2025，Vol.42Issue(3)：113-118,6.

中国动物检疫2025，Vol.42Issue(3)：113-118,6.DOI:10.3969/j.issn.1005-944X.2025.03.018

肉鸡屠宰及零售环节沙门氏菌污染的定性和定量检测方法比较

Comparison of Qualitative and Quantitative Detection for Salmonella Contamination in the Links of Broiler Slaughter and Retail

张思宇 ¹高玉斌 ²曲志娜 ²王君玮 ²赵格 ³黄保续 ⁴刘俊辉 ²王娟 ²王琳 ³刘娜 ³张喜悦 ²宋时萍 ²赵建梅 ²黄秀梅²

作者信息

1. 东北农业大学动物医学学院,黑龙江哈尔滨 150030||中国动物卫生与流行病学中心,农业农村部畜禽产品质量安全风险评估实验室(青岛),山东青岛 266032
2. 中国动物卫生与流行病学中心,农业农村部畜禽产品质量安全风险评估实验室(青岛),山东青岛 266032
3. 中国动物卫生与流行病学中心,农业农村部畜禽产品质量安全风险评估实验室(青岛),山东青岛 266032||农业农村部动物生物安全风险预警及防控重点实验室(南方),青岛市动物生物安全重点实验室,山东青岛 266032
4. 东北农业大学动物医学学院,黑龙江哈尔滨 150030
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摘要

Abstract

In order to find out an optimal method for detecting the contamination rate and load of Salmonella in chicken,cotton swabs as well as washing liquor samples of carcasses/products were collected to compare the detection rate of Salmonella using cotton swab culture,carcass/product washing liquor culture,maximum probable number(MPN)method of carcass/product washing liquor and the combination of MPN method of carcass/product washing liquor and real-time fluorescent PCR(qPCR-MPN),respectively,and the loads of Salmonella were also compared using the last two methods.Qualitative detection results showed that the detection rate by qPCR-MPN method was the highest(84%),followed by MPN method of carcass/product washing liquor(80%),carcass/product washing liquor culture(60%)and cotton swab culture(28%),with statistically significant differences among the above methods(P＜0.05);taking the carcass/product washing liquor culture as a standard,both qPCR-MPN and MPN method of carcass/product washing liquor were 100%sensitive and＞80%consistent,but the latter was more specific.Quantitative detection results showed that more amount of Salmonella was detected by the qPCR-MPN method,compared to the MPN method of carcass/product washing liquor.In conclusion,the MPN method of carcass/product washing liquor was preferred for both qualitative and quantitative data;the qPCR-MPN method was highly consistent with the MPN method of carcass/product washing liquor,detecting more amount of Salmonella,but the risk of false positive should be noted when it was used for quantitative detection.

关键词

沙门氏菌/肉鸡/定性检测/定量检测/方法比较

Key words

Salmonella/broiler/qualitative detection/quantitative detection/method comparison

分类

农业科技

引用本文复制引用

张思宇,高玉斌,曲志娜,王君玮,赵格,黄保续,刘俊辉,王娟,王琳,刘娜,张喜悦,宋时萍,赵建梅,黄秀梅..肉鸡屠宰及零售环节沙门氏菌污染的定性和定量检测方法比较[J].中国动物检疫,2025,42(3):113-118,6.

基金项目

国家重点研发计划项目(2022YFC2303900) （2022YFC2303900）

青岛市科技惠民示范专项项目(24-1-8-xdny-21-nsh) （24-1-8-xdny-21-nsh）

中国动物检疫

ISSN：1005-944X

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